Glucohydrolase

M. Hrmova, R. de Gori, B. J. Smith, A. Vasella, J. N. Varghese, and G. B. Fincher, Three-dimensional structure of the barley p-D-glucan glucohydrolase in complex with a transition state mimic, J. Biol. Chem., 279 (2004) 4970 1980. 

B. Lesur, J.-B. Ducep, M.-N. Lalloz, A. Ehrhard, and C. Danzin, New deoxynojirimycin derivatives as potent inhibitors of intestinal a-glucohydrolases, Bioorg. Med. Chem. Lett., 3 (1997) 355-360. 

L-arabinose, have also been reported to be in direct linkage with a protein. L-Arabinose may also be linked to L-serine and L-threonine in a hyaluronic acid-polypeptide complex from ox vitreous-humor.750 D-Mannose is reported to be glycosidically linked in a glucoamylase [(l->4)-a-D-glucan glucohydrolase, EC 3.2.1.3] from Aspergillus... 

Glucan Glucohydrolases Action Mechanisms and Enzyme-Carbohydrate Complexes... 

The sedimentation rate of the almond glucohydrolase on density gradient tests was slightly smaller than the rate for glucoamylase. Apparently the almond glucohydrolase is a somewhat smaller molecule. 

The rate data on the hydrolysis of different oligosaccharides by the almond glucohydrolase are shown in Table II. These data can be interpreted to indicate that the active site for the enzyme is composed of two subsites. Apparently the active site is so located on the enzyme that additional glucose residues in the substrate interfere with the binding of the substrate to the active site. 

Enzymes involved in rp endo- 3-l,4-glucanase, exo-P-l,4-cellobiohydrolase. Enzymes involved in r2 exo-P-l,4-cellobiohydrolase, exo-P-l,4-glucan glucohydrolase. Enzymes involved in r3 P-bludosidse or cellobiases. 

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