Thioglucoside glucohydrolase

HUSEBYE, H., CHADCHAWAN, S., WINGE, P., THANGSTAD, O.P., BONES, A.M., Guard cell- and phloem idioblast-specific expression of thioglucoside glucohydrolase 1 (myrosinase) in Arabidopsis., Plant Physiol., 2002,128,1180-1188. 

PETROSKI, R.J., TOOKEY, H.L., Interactions of thioglucoside glucohydrolase and epithiospecifier protein of cruciferous plants to form 1-cyanoepithioalkanes., Phytochemistry, 1982,21, 1903-1905. 

MacLeod, A.J. and Rossiter, J.T. (1986) Isolation and examination of thioglucoside glucohydrolase from seeds of Brassica napus. Phytochemistry, 25, 1047-52. 

Thangstad, O.P., Winge, R, Husebye, H. and Bones, A. (1993) The thioglucoside glucohydrolase (myrosinase) gene family in Brassicaceae. Plant Mol Biol, 23, 511-24. 

Fig.1. Glucosinoiate. Conversion of a glucosinoiate to the corresponding isothiocyanate by the action of thioglucoside glucohydrolase.

GSLs are not bioactive until they have been enzymatically hydrolyzed to various bioactive breakdown products by the endogenous plant enzyme called m5Tosinase (thioglucoside glucohydrolase). Upon cell... 

In plant tissue, glucosinolates are accompanied by the enzyme myrosinase (thioglucoside glucohydrolase), which catalyses their decomposition. Myrosinase is a globular glycoprotein with a relative molecular weight of 140 kDa, consisting of several isoenzymes. 

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