Gibberella fujikuroi fermentation

Gibberellic acid production was studied in different fermentation systems. Free and immobilized cells of Gibberella fujikuroi cultures in shake-flask, stirred and fixed-bed reactors were evaluated for the production of gibberellic acid (GA3). Gibberellic add production with free cells cultured in a stirred reactor reached 0.206 g/L and a yield of 0.078 g of GA3/g biomass. 

Fed-batch operations can also be operated where soluble nutrients are added to the bed. They can be sprayed onto the bed as a fine mist [89, 90, 129], or simply mixed in with the substrate, such as has been done with soluble starch during a fed-batch culture of Gibberella fujikuroi on wheat bran [130]. This technique of fed-batch operation is appropriate for soluble nutrients which cause substrate inhibition if added in sufficient amounts for the whole fermentation at the start of the fermentation. 

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