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Massively parallel signature sequencing

Brenner S et al. Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays. Nature Biotechnol 2000 18 630-634. [Pg.112]

Erdner, D. L., and Anderson, D. M. (2006). Global transcriptional profiling of the toxic dinoflageUate Alexandriumfundyense using massively parallel signature sequencing. BMC Genomics 7(88). [Pg.1333]

In massive parallel signature sequencing (13), 3 sequences of each transcript are isolated using biotinylated primer in the cDNA synthesis and are cleaved with a restriction enzyme that generates a cohesive end. Next, the 3 signature sequences are... [Pg.1846]

Brenner S, Johnson M, Bridgham J, Golda G, Lloyd DH, Johnson D, Luo S, McCurdy S, Foy M, Ewan M. Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays. Nat. Biotechnol. 2000 18 630-634. [Pg.1852]

Oudes AJ, Roach JC, Walashek LS, Eichner LJ, True LD, Vessella RL, Liu AY (2005) Application of Affymetrix array and Massively Parallel Signature Sequencing for identification of genes involved in prostate cancer progression. BMC Cancer 5 86... [Pg.427]

Products/technologies Massively parallel signature sequencing technology has the power to examine the genetic activity of a single cell. [Pg.256]

There are direct measures of gene expression, but these tend to be limited by expense. They include TaqMan assays, which can measure the expression of several hundred genes directly, massively parallel signature sequencing (MPSS), expressed sequence tag (EST) counts, and serial analysis of gene expression (SAGE). Microarrays measure gene expression indirectly, and dye effects such as the dye bias of Cy3/Cy5 require lowess correction. [Pg.127]


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