Genomic techniques sequencing

Fluorescently labeled DNA probes can be used for detection, localization, or quantification of target DNA sequences. In situ hybridization mapping of genomic DNA sequences can be done using fluorescent probes to target particular regions within chromosomes. Called FISH for fluorescent in situ hybridization, the technique is used... 

The expected availability of genome length sequences in the near future requires the implementation of new techniques for the analysis of genome level evolutionary events. Based on the... 

With both single- and double-surface techniques detection of single-base mismatches (point mutations) is possible. At present principles of the electrochemical detection of the DNA hybridization event and determination of various genomic nucleotide sequences, after the PCR amplification of DNA, are known. On the other hand, a lot of work has to be done to develop and optimize inexpensive and simple electrochemical DNA sensors with performance comparable to that of DNA arrays based on optical detection. Determination of nucleotide sequences without DNA amplification represents a great challenge. To reach this goal it will be necessary to... 

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