Genomic libraries screening methods

These libraries are used in many screening procedures and many transgenic proteins now routinely available were obtained by their use. Although in some applications the use of cDNA and genomic libraries has been superceded by other methods, particularly those based on PCR, they are still used to advantage in many applications. 

Two implementations of genomes, a binary and a decimal (figure 2), were compared to study the dependence on a DNA-like and chemist-like crossover and mutation. Further variables were population size, mutation rate and the crossover strategy. In order to eliminate the stochastic differences between different GA runs, each parameter set was used in 100 parallel runs and the averages and standard deviations for the various results were calculated and displayed on the following figures. All results were compared with a "random" screening method that was simulated by a repeated random selection of new products from the library. 

Once the genomic library has been generated it is necessary to screen for the gene of interest within a population of thousands of recombinant clones. The choice of screening method will very much depend on the availability of reagents and the information on the target gene to be isolated. 

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