Genome Reduction

subtilis cell and SM is a selection marker on the chromosome of the mutant 6. subtilis protoplast. (2) Intensive crossing over between the chromosomes of the donor and recipient bacteria. (3) Selection and counterselection provide transformants with thoroughly mixed-up genetic traits of the recipient and the donor strains. 

---

Gil, R., Sabater-Munoz, B., Latorre, A., Silva, F. J., and Moya, A. (2002). Extreme genome reduction in Buchnera spp toward the minimal genome needed for symbiotic life. Proc. Natl. Acad. ScL USA, 99,4454-8. 

The success of such a genome reduction program depends on (i) the accurate identification of the dispensable genomic regions that are not required for essential cellular functions or fermentative production of proteins or metabolites ... 

Morimoto, T. et al (2008) Enhanced recombinant protein productivity by genome reduction in Bacillus subtilis. DNA Res., 15 (2), 73-81. 

V., and Takors, R. (2015) Genome reduction boosts heterologous gene expression in Pseudomonas putida. Microb. Cell Fact., 14, 23. 

The Chassis Concept Biotin Prototrophy and Genome Reduction... 

Metabolic engineering aims at improving yields, titers, and productivities. The use of inexpensive media components for fermentation without the need to add cost-intense supplements is desirable. Genome reduction is believed to be important for establishing a lean, but versatile production strain as chassis. 

The Chassis Concept Biotin Pratatrophy and Genome Reduction 375... 

---