Genome mining

Zhu, D., Mukherjee, C., Biehl, E.R. and Hua, L. (2007) Discovery of a mandelonitrile hydrolase from Bradyrhizobium japonicum USDA110 by rational genome mining. Journal of Biotechnology, 129, 645-650. 

Lautru S, Deeth RJ, Bailey LM, Challis GL (2005) Discovery of a New Peptide Natural Product by Streptomyces coelicolor Genome Mining. Nature Chem Biol 1 65... 

In a different context the catchword genome mining occurs this refers to the possibility of searching the available genomes for suitable or interesting ORFs (open reading frames), which can be used as a basis of cloning. 

As briefly mentioned above, the accumulated knowledge and the discoveries connected with genome mining and accessing the metagenome of soil-borne... 

Challis GL (2008) Genome Mining for Novel Natural Product Discovery. J Med Chem 51 2618... 

Cone C, Challis GL (2007) Heavy Tools for Genome Mining. Chem Biol 14 7... 

Lin X, Hopson R, Cane DE (2006) Genome Mining in Streptomyces coelicolor Molecular Cloning and Characterization of a New Sesquiterpene Synthase. J Am Chem Soc 128 6022... 

Lautra S, Deeth RJ, Bailey LM, ChalUs GL. Discovery of a new peptide natural product by Streptomyces coelicolor genome mining. Nat. Chem. Biol. 2005 1 265-269. 

Based on this MS dissection of the native substrates and chemical modifications, a related or even identical structure of the unknown natural product can be synthesized and tested in terms of bioactivity and physiological target. The result from the in vitro reconstitution should be confirmed by another genome mining... 

Takayama et al., 1999 Thress et al., 2001), most of which have been identified by two-hybrid screens and genome mining of human cDNA libraries (Takayama et al., 1999 Thress et al., 2001). All homologs by definition contain a Bag domain, but otherwise show a high degree of difference in their domain composition e.g., four of these homologs do not code for any of the other domains found in Bag-1 (Fig. 5). Bag-1... 

D. Krug, N. S. Cortina, and R. Muller, unpublished data). As these few examples illustrate, genome mining in myxobacteria appears to have a promising future. 

Role of Bioinformatics in Natural Product Discovery by Genome Mining 435... 

Experimental Strategies forthe Isolation of New Natural Products by Genome Mining 442... 

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