Genome editing tools

Recently, a remarkable series of new companies, four within 4 years, have been founded in order to exploit a new tool, a technology to explore genomics and genome editing tools of remarkable precision two founded by the inventors Jennifer Doudna and Emmanuelle Charpentier. These companies created an investment of 345 miUion within that short time. However, in the area of personalized medicine, some have ethical concerns with respect to germline alterations (as opposed to somatic tissue alterations see also the end of Section 1.5) [93-95]. 

In this chapter we revise some of the tools and fundamentals of synthetic biology, such as the use of engineering principles, genome editing tools and metabolomics. In addition, we discuss the potential of synthetic biology for medical applications in the prevention, diagnosis and treatment of diseases. 

Cai, M., Yang, Y, 2014. Targeted genome editing tools for disease modehng and gene therapy. Curr. Gene Then 14, 2-9. 

Sim, N. and H. Zhao, Transcription activafor-Uke effector nucleases (TALENs) A highly efficient and versatile tool for genome editing. Biotechnol. Bioeng., 2013. 110(7) 1811-1821. 

The fast development of the new tools discovered in the recent years for genome editing, as well as emergent approaches to assemble DNA parts will certainly foster the development of new bioinspired materials for medical applications. 

SRI International Pathway Tools software supports creation, editing, querying, visualization, analysis, and publishing of Pathway/Genome Database (http // bioinformatics.ai.sri.com/ptools/)... 

The sixth edition oi Biochemistry offers three chapters that present the tools and techniques of biochemistry Exploring i roteins and Proteomes" (Chapter 3), Exploring Genes and Genomes (Chapter 4), and Exploring Evolution and Bioinformatics (Chapter 6). Additional experimental techniques are presented throughout the book, as appropriate,... 

Martinez-Garcia, E. and de Lorenzo, V. (2012) Transposon-based and plasmid-based genetic tools for editing genomes of gram-negative bacteria. Methods Md. Biol, 813, 767-TPS. 

In 1994, the first edition of this book was published. Since then there have been several almost revolutionary advances in the technology of x-ray crystallography. Among them are the x-ray source, new instruments and faster computer programs. The accomplishments of the Human Genome Project pushes a vigorous pursuit for the determination of the structures of both nucleic acid and proteins. X-ray crystallography is the number 1 tool, followed by NMR. 

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