Genome analysis description

Recent advances in micro-manipulation and hybridization, fluorescence labeling, and large-scale sequencing projects have led to new methods of expression profiling and genome analysis, loosely termed microarray analysis. This is an oblique description for a number of methods that utilize hybridization of labeled complex RNA probes to immobilized nucleotides arrayed at high density on a solid substrate to provide quantifiable data on relative expression levels. 

Several different approaches to microarray construction have been developed, including oligonucleotide- or DNA-based arrays on glass slides or nylon membranes (10). Here, we describe a robust CGH protocol based on the use of a PCR-generated microarray (6). Detailed descriptions of genomic DNA template preparation, labeling, microarray hybridization, and data analysis are provided. 

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