Genetic engineering approaches

Once isolated, the natural H2 producers can be optimized by conventional mutagenesis, and they should be studied so that we can understand those features that make them the best H2 producers. This characterization would involve the analysis of metabolic fluxes (Stephanopoulos and Sinskey 1993 Schuster et al. 1999) and molecular genetics. It would result in new, previously unknown adaptations necessary for improved H2 production, and could provide information on the most important mutations that are required to obtain excellent H2 producers. Information obtained from these experiments should be used in genetic engineering approaches for optimizing H2 producers. Moreover, excellent H2 producers should be used in bioengineering approaches. 

Muhitch MJ A genetic engineering approach to lowering fumonisin levels in maize kernels. Proceedings of the USDA-ARS FusariumiVmaomsm Workshop, Beltsville 1995, p 27. 

Jimenez-Flores and Richardson (36) have discussed a strategy for the rational modification of proteins using a genetic engineering approach. They have suggested that the rapid advances that have been... 

Lucca P, HurreU R, Potrykus I (2001) Genetic engineering approaches to improve the bioavailability and the level of iron in rice grains. Theor Appl Genet 102 392-397 Machado ME, Saraiva JM (2005) Thermal stability and activity regain of horseradish peroxidase in aqueous mixtures of imidazolium-based ionic liquids. Biotechnol Lett 27 1233-1239 Maeda H, Chen L, Tsao G (1979) Glucoamylase immobilized on DEAE-ceUulose beads in column taction. J Ferment Technol 57 238-243... 

Among the strategies used for increasing the produetion of medicinal componnds it can be mentioned the media design, elicitation and the genetic engineering approach. 

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