Genetic analysis of yeast

Naumov, G. I., Masneuf, I., Naumova, E. S., Aigle, M., and Dubourdieu, D. (2000). Association of Saccharomyces bayanus var. uvarum with some French wines Genetic analysis of yeast populations. Res. Microbiol. 151, 683-691. 

Gallego, F. J., Perez, M. A., Nunez, Y., Hildago, P. (2005). Comparison of RAPDs, AFLPs and SSR marker for genetic analysis of yeast strains of Saccharomyces cerevisiae. Food... 

Zhu H et al. Analysis of yeast protein kinases using protein chips. Nature Genet 2000 26 283-289. 

Zhu, H., Klemic, J.F., Chang, S., Bertone, R, Casamayor, A., Klemic, K.G., Smith, D., Gerstein, M., Reed, M.A., and Snyder, M., Analysis of yeast protein kinases using protein chips, Nature Genetics, 26, 283-289, 2000. 

This example inspired searches for radiation sensitive mutants in yeast. The way was led by Nakai and Matsumoto (1967) who isolated one mutant, UV which was very sensitive to ultraviolet and a second, Xj sensitive to X-rays. They went one step further than isolation and survival curves by making the double-mutant and showing that like double mutants of recA and uvr A in E. coli it was much more sensitive to UV than either single mutant alone. This was the first demonstration of the existence of more than one type or pathway of DNA repair of UV damage in yeast, and inspired the later work of Game and Cox (1972 1973 1974), Brendel and Haynes (1973) and Louise Prakash (1993) in the genetic analysis of pathways of repair in yeast. This led to the classification of the many mutant loci into epistasis groups, which are defined as those mutants which, when combined in the same strain, are no more UV-sensitive than the most sensitive of the two when alone. 

DD Shoemaker, DA Lashkari, D Morris, M Mittmann, RW Davis. Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular barcoding strategy. Nature Genet 14 450-456, 1996. 

Antunovics, Z., Csoma, H., and Sipiczki, M. (2003). Molecular and genetic analysis of the yeast flora of botrytized Tokaj wines. Bull. OIV 76, 380-397. 

Jimenez, J., Bemtez, T. (1987). Genetic analysis of highly ethanol-tolerant wine yeasts. Curr. Gen., 12, 421 28. 

Zhu H, Klemic IF, Chang S, Bertone P, Casamayor A, Klemic KG, Smith D, Gerstein M, Reed MA, Snyder M. Analysis of yeast protein kinases using protein chips. Nat. Genet. 2000 26 283-289. Horiuchi KY, Wang Y, Diamond SL, Ma H. Microarrays for the functional analysis of the chemical-kinase interactome. J. Biomol. Screen. 2006 11 48-56. 

Carro, D. and Pina, B. 2001. Genetic analysis of the karyotype instability in natural wine yeast strains. Yeast 18,1457-1470. 

Takahashi, T. 1978. Genetic analysis of a German wine yeast. Bull. Brew. Sci. 24, 39-47. Thomsson, E., Gustafsson, L., and Larsson, C. 2005. Starvation response of Saccharomyces... 

As is the case in DNA replication, most of what we know about DNA repair mechanisms comes from the study of E. coli. However, recent studies of DNA repair in eukaryotes, primarily genetic analysis in yeast and genetic studies of human diseases, have revealed that similar processes are also found in these organisms. Table 23.1 lists the four basic types of DNA repair systems in E. coli and the proteins which have been characterized. The human genes for the corresponding proteins are also shown in cases where they have been identified. 

PFG can vastly simplify genetic analysis as demonstrated in its application to analysis of yeast chromosomal translocations. 

Fig. 10. Genetic analysis of aminoglycerophospholipid transport in eukaryotes. The transport of PS synthesized in the ER is regulated by PSTA and PSTB genes. The acronym stands for PS transport (either A or B pathways). Likewise, the transport of PE synthesized in the mitochondria or Golgi/vacuole is proposed to be regulated by PEEA and PEEB genes. The acronyms stand for PE export (either A or B pathways). Both known and proposed mutations along the metabolic and transport pathways appear in lower case italics. The table summarizes the mutants, genes, and proteins that have been identified in Chinese hamster ovary (CHO) cells and yeast. Other abbreviations pss, PS synthase psd, PS decarboxylase pern, PE methyltransferase Cho, choline Etn, ethanolamine Ser, serine.

H. Zhu, J.F. Klemic, S. Chang, P. Bertone, A. Casamayor, K.G. Klemic, D. Smith, M. Gerstein, M.A. Reed, and M. Snyder, Analysis of yeast protein kinases using protein chips. Nat. Genet. 26, 283—289... 

Genetic analysis in yeast confirms the importance of TAFs, as all (with the exception of yeast TAF14, a protein found in several transcription-related complexes) are essential for viability. However, the requirement for individual TAFs in general Pol II transcription is still unclear. Various... 

McKee, R. H., and Lawrence, C. W. (1979a). Genetic analysis of gamma-ray mut ene-sis in yeast I. Reversion in radiation-sensitive strains. Genetics 93, 361-373. 

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