Genetic analysis fundamentals

Although SGE, CEPS, and microchip analysis are of fundamental importance for genetic analysis, it is important to note that several new promising technologies already exist that allow DNA sequencing without using the classic Sanger biochemistry principle, capillaries, or microchannels (3,17). 

Encouraged by this spectral reproducibility, we focused our efforts on the particularly challenging problem of distinguishing bacterial strains by MALDI MS. We developed a modified correlation approach22 that relies on two fundamental qualities of bacterial mass spectra. First, because different bacterial strains of the same species have substantial, if not complete, genetic overlap, most of the protein masses observed with two different strains will be identical. This feature limits the value of the biomarker approach that is commonly used to differentiate bacteria species. Second, as just noted, closely controlled sample preparation and mass analysis procedures can result in highly reproducible results.22 The modified correlation approach takes advantage of subtle, yet reproducible, differences in mass spectra obtained from dif-... 

Leung YF, Cavalieri D. 2003. Fundamentals of cDNA microarray data analysis. Trends in Genetics 19 649. 

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