Genetic analyses design

Polymerase chain reaction is particularly useful for genetic analysis because both amplification and primer specific isolation of gene fragments occur simultaneously. Allele-specific amplification can be employed to detect a single base-pair mutation through the use of a specially designed primer which is complementary to... 

S. cerevisiae mutants blocked in the conversion of 4-HB to (Figure 19) are unable to grow on oxidizable carbon sources. An extensive genetic analysis of these mutants led to the identification of the various steps involved in the conversion of 4-HB to Nine complementation groups designated (COQJ—COQ9) were identified among the mutants unable to form These mutants proved to be a very valuable resource... 

A third high-affinity iron-uptake system mediates the accumulation of iron complexed with citrate. Growth of . coli in the presence of citrate induces the production of an outer membrane protein (M 80,500). Genetic analysis of the iron citrate transport system of E. coli located the genes controlling the system, designated fee. Subsequently the /ec locus was subdivided into fecA and /ecS, the former coding for the outer membrane protein. 

Vasilyeva, E., B. Lam, Z. Fang, M.D. Minden, E.H. Sargent, and S.O. Kelley. 2011. Direct genetic analysis of ten cancer cells Tuning sensor structure and molecular probe design for efficient mRNA capture. Angew. Chem. Int. Ed. 50 4137 141. 

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