General conditions of electrophoresis under high hydrostatic pressures

General conditions of electrophoresis under high hydrostatic pressures 

The ionization of weak acids varies with both the temperature and the pressure. This process is accompanied by electrostriction of water around the charges that are created. The pressure dependence of the pH is 

For example, due to the large negative volume change of H2P04 upon ionization, the phosphate buffer undergoes a pH shift of -0.44 for AP = 1 kbar at 25 °C. On the other hand, the pH values of buffers made with conjugate acids of neutral bases such as Tris-HCl and imidazole-HCl are nearly pres- 

Polyacrylamide gels with a total acrylamide content of 4-9 per cent T (in per cent) = [acrylamide -I- bismethylene acrylamide]/100) and C = 3-4 per cent (C = [bismethylene acrylamide]/r) are prepared in the standard way using ammonium persulfate as the initiator and tetramethylethylenediamine (TEMED) as the catalyst for gel formation (for general preparation of poly- 

Standard discontinuous alkaline buffer system for polyacrylamide-gei electrophoresis of low-isoelectric-point proteins 

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