Gene-splicing

Splidng (1) Gene splicing manipulations, the object of which is to attach one DNA molecule to anodier (2) RNA splicing removal of inh ons fiom mRNA precursors. 

Hegele, R. A., Anderson, C., Young, T. K. Connelly, P. W. (1999). G-protein beta3 subunit gene splice variant and body fat distribution in Nunavut Inuit. Genome Res., 9, 972-7. 

H3 receptors are a family of GPCRs produced by gene splicing and are linked to Gi/0 257... 

The TBXA2R gene splice variants result, therefore, in protein structures with distinct functions. An amino acid substitution that is deleterious in one splice isoform, however, may only be a polymorphic marker in another. This phenomenon... 

Gene splicing provides a path to protein diversity... 

The steps involved in gene splicing, emphasizing the chemical nature of the individual steps, are as follows ... 

Why is E. coli most often used in gene splicing ... 

Although the role of the terpenes in the plants that produce them is still a matter of debate, evidence of an anti-herbivore function for many of them is accumulating. Modern DNA technology, gene splicing, and cloning techniques will undoubtedly make it possible to incorporate suitable defensive allelochemlcals into selected crop plants in order to minimise crop devastation by insect herbivores. However, it is necessary to understand the fund-... 

Horton, R. M, Cai, Z., Ho, S. N., and Pease, L. R. (1990) Gene splicing by overlap extension tailor made genes using the polymerase chain reaction Biotechniques 8, 528-535. 

Horton, R M., Hunt, H. D., Ho, S N., Pullen, J K., and Pease, L R. (1989) Engineering hybrid genes without the use of restriction enzymes, gene splicing by overlap extension. Gene 77, 61-68... 

Within the last ten years phenomenal advances in the field of genetic engineering have taken place. It is hoped, but not yet demonstrated, that gene splicing may lead to major advances in the production of alcohol, but carefully controlled use of conventional microorganisms is now the best potential route to good economics. 

Fig. 20.1 Leptin receptor isoforms. Leptin receptor isoforms (Ob-Ra to Ob-Re) are the result of alternative gene splicing. Ob-Rb is considered to be the active isoform for carrying out downstream signaling. The extracellular region of the receptor is comprised of two CK (cytokine receptor domain) and three F3 (fibronectin domain) domains. The extracellular domain is followed by the transmembrane domain. The intracellular region consists of B1 (box 1) and B2 (box 2) for JAK2 binding and STAT (signal transducers and activators of transcription) binding domain.

Sun L, Yu C, Irudayaraj J (2008) Raman multiplexers for alternative gene splicing. Anal Chem 80 3342-3349... 

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