Gene regulation mammalian cells

Shamanski FL, Orr-Weaver TL 1991 The Drosophila plutonium and pangu genes regulate entry into S phase at fertilization. Cell 66 1289—1300 Sherr CJ 1993 Mammalian G1 cyclins. Cell 73 1059-1065... 

T. Cremer, and C. Cremer, Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat. Rev. Genet. 2, 292-301 (2001). 

Yao F, Svensjo T, Winkler T et al (1998) Tetracycline repressor, tetR, rather than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression in mammalian cells. Himi Gene Ther 9 1939-1950... 

Bae K, Lee C, Sidote D, Chuang KY, Edery I 1998 Circadian regulation of a Drosophila homolog of the mammalian Clock gene PER and TIM function as positive regulators. Mol Cell Biol 18 6142-6151... 

Hepatic peroxisome proliferation depends on a nuclear receptor, PPARa, to mediate these responses in mice, based on lack of response to peroxisome proliferators in PPARa-deficient mice. In one study with another peroxisome proliferator, WY-14,643, carcinogenesis was shown to be dependent on the same receptor. Oral administration of di(2-ethylhexyl) phthalate failed to elicit markers of peroxisome proliferation in PPARa-deficient mice, while the same treatment elicited this response in normal mice. Metabolites of di(2-ethylhexyl) phthalate caused activation of PPARa-mediated gene expression in mammalian cell co-transfection assays. Differences between responsive rodents and humans in various aspects of PPARa-mediated regulation of gene expression are consistent with the lack of activity of di(2-ethylhexyl) phthalate metabolites in hepatocyte cultures from 12 people studied to date. 

Guhaniyogi, J. and Brewer, G. (2001) Regulation of mRNA stability in mammalian cells. Gene, 265, 11-23. 

Ward GA, Stover CK, Moss B, Fuerst TR (1995), Stringent chemical and thermal regulation of recombinant gene expression by vaccinia virus vectors in mammalian cells, Proc. Natl Acad. Sci. U S A 92 6773-6777. 

Lin et al. [149] have recently reported the synthesis of 2-(4,5-dimethoxy-2-nitrobenzyl)-20-hydroxyecdysone (6-6), which itself has a very low affinity for ecdysteroid receptors expressed in mammalian 293T cells, but can be photolysed at 348 nm to release 20E, which then activates the receptor-regulated reporter gene. With this system, it will be possible not only to control the temporal activation of transfected genes, but also their spatial activation. The authors recognise that the effectiveness of this system can be considerably improved in future by derivatising an ecdysteroid (poA or muA) which is more active in ecdysteroid-responsive mammalian cells. 

---