Gene hybridization

Bruns, M. A., Fries, M. R., Tiedje, J. M., and Paul, E. A. (1998). Functional gene hybridization patterns of terrestrial ammonia-oxidizing bacteria. Microb. Ecol 36, 293—302. 

Figure 5. Effect of acetate on CA transcript abundance. Total RNA was isolated from air grown cells (lane 1) and from cells exposed to 10 mM Na acetate in the light for 6 h (lane 2) and 15 h (lane 3). Following electrophoresis on a 1.1% agarose gel, the RNA was transferred to Biodyne A nylon membrane (ICN) and probed with the P-labelled CA gene. Hybridization was performed at 42 C and 50% formamide for 18 h. The blots were subsequently washed for three 5 min periods in 2 X SSC and 0.1% SDS at room temperature followed by a 20 min wash at 42 C in 0.1 x SSC and 0.1% SDS. The size of the CA transcript is indicated and was determined by comparison with a synthetic RNA ladder (BRL Corp.)...

The pobAB from S. griseus IMRU 3570 has been used as a probe to find new aromatic polyene-producing Streptomyces strains. The pobAB gene hybridizes with 6 out of 16 Streptomyces strains, and those strains chat hybridize turned out to be aromatic polyene... 

On the other hand, no polymerization of the biotinylated C-base can occur on the homocygotic gene hybridized to the sensing interface, and thus no increase in the interfacial electron-transfer resistance is observed upon interaction with polymerase/biotinylated dCTP and an attempt to precipitate (12). 

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