Gene expression studies applications

Gellissen G, Hollenberg C P (1997). Application of yeasts in gene expression studies A comparison of Saccharomyces cerevisiae, Hansenula polymorpha and Kluyveromyces lactis—a review. Gene 190 87-97. 

Both these strategies create knock-in situations the off-on switch results in a reporter gene knock-in behind the endogenous CPTl promoter after inactivation of CPTl, while the on-off approach attaches the reporter to active CPTl and allows gene expression studies in the first generation of mice already. For these reasons our first efforts focus on the latter strategy. Much will depend on the flexibility of CPTl enzyme function, and also on the applicability and sensitivity of GFP in these studies. 

Low space requirements of devices Integration of functions like reverse transcription process (for gene expression studies), on-line measurement of double-strand DNA concentration, or real-time measurement by multi-sensor arrangements Hence, miniaturized PCR devices are very interesting for a broader application of PCR in laboratories as well as for point-of-care diagnostics, screenings, or investigations for food safety and environmental protection [7, 8]. 

Cassar-Malek, I., B. Picard, C. Bernard and J.F. Hocquette, 2008. Application of gene expression studies in livestock production systems a European perspective. Austr. J. Exp. Agric. 48, 701-710. 

Hicks DG, Longoria G, Pettay J, et al. In situ hybridization in the pathology laboratory general principles, automation, and emerging research applications for tissue-based studies of gene expression. J. Mol. Histol. 2004 35 595-601. 

Biomolecular MS and in particular MALDI-TOF-MS (see Sections 2.1.22 and 2.2.1) permit the routine analysis of oligonucleotides up to 70-mers, intact nucleic acids, and the direct detection of DNA products with no primer labels with an increase in analysis speed and mass accuracy especially in contrast to traditional DNA separation techniques such as slab gels or capillary electrophoresis. Applications focus on the characterization of single nucleotide polymorphisms (SNPs) and short tandem repeats (STRs). Precise and accurate gene expression measurements show relative and absolute numbers of target molecules determined independently of the number of PCR cycles. DNA methylation can be studied quantitatively. 

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