Gene expression markers

Thompson KL, Afshari CA, Amin RP, Bertram TA, Car B, Cunningham M, Kind Cl, Kramer JA, Lawton M, Mirsky M, Naciff JM, Oreffo V, Pine PS, Sistare FD. Identification of platform-independent gene expression markers of cisplatin nephrotoxicity. Environ Health Perspect 2004 112(4) 488-94. 

Ven LT (2014) Gene expression markers in the zebrafish embryo reflect a hepatotoxic response in animal models and humans. Toxicol Lett 230, 48-56. 

Chalfie, M., et al., 1994. Green fluorescent protein as a marker for gene expression. Science 263 802-805. 

Herzog, A, U Siler, V Spitzer et al. 2005. Lycopene reduced gene expression of steroid targets and inflammatory markers in normal rat prostate. FASEB J 19 272-274. 

It is well accepted that MDMA produces 5-HT depletions in rat CNS, but much less attention has been devoted to the effects of MDMA on established markers of neurotoxicity such as cell death, silver-positive staining, and reactive gliosis. Support for the hypothesis of MDMA-induced axotomy relies heavily on immunohistochemical analysis of 5-HT levels, which could produce misleading results if not validated by other methods. For example, MDMA-induced loss of 5-HT could be due to persistent adaptive changes in gene expression or protein function, reflecting a state of metabolic quiescence rather than neurotoxic damage. Table 7.3 summarizes the effects of MDMA on hallmark measures of neurotoxicity. 

Rininger JA et al. Differential gene expression technologies for identifying surrogate markers of dmg efficacy and toxicity. DDT 2000 5 560-568. 

Higher expression levels were associated with the encapsulated DNA than with the administration of naked DNA in vitro, but standard liposomal transfection yielded the highest level of gene expression. In vivo, however, the encapsulated DNA exhibited higher levels of sustained expression of a marker gene at 28 days, than that associated with naked DNA or DNA delivered with liposomes. Thus, this study indicated the potential for DNA encapsulated in degradable nanospheres to elicit sustained gene expression in vivo. 

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