Gene delivery vascular smooth muscle cells

DNA binding moiety has been synthesized and evaluated as a receptor-mediated gene delivery system. The system was able to transfect about 30% of corneal endothelial cells of rabbit, pig and human in the presence of chloroquine (Shewring et al., 1997). Associated with the fusogenic peptide of influenza, this peptide transfected 25-30% of primary cultures of vascular smooth muscle cells of man, rabbit and rat (Collins et al., 2000 Li et al., 2000). The molossin-based gene delivery system represents an interesting system in transplantation because the molossin peptide does not bind to vascular endothelium and pancreatic islets. 

Li, J.-M., Collins, L., Zhang, X.-H., Gustafsson, K. and Fabre, J.W. (2000) Efficient gene delivery to vascular smooth muscle cells using a nontoxic, synthetic peptide vector system targeted to membrane integrins a first step toward the gene therapy of chronic rejection. Transplantation, 70, 1616-1624. 

Murakami H., Yayama K, Miao RQ, et al. Kallikrein gene delivery inhibits vascular smooth muscle cell growth and neointima formation in the rat artery after balloon angioplasty. Hypertension 1999 34 164-170. 

March KL, Madison JE, Trapnell BC. Pharmacokinetics of adenoviral vector-mediated gene delivery to vascular smooth muscle cells modulation by poloxamer 407 and implications for cardiovascular gene therapy. Hum Gene Ther 1995 6 41-53. 

The primary amines located on the surface of PAMAM dendrimer were conjugated with L-arginine [104]. Polyplexes composed of L-arginine-grafted PAMAM/DNA showed increased gene delivery potency compared to native PAMAM dendrimer and L-lysine-grafted PAMAM, as evaluated for 293, HepG2, and Neuro 2A cells and in primary rat vascular smooth muscle cells. 

Gomez-Navarro J, Pierce GF, Curiel DT, Douglas JT. Taigeting adenoviral infection with basic fibroblast growth ctor enhances gene delivery to vascular endothelial and smooth muscle cells. Tumor Taigeting 1998 3 156-168. 

---