Gene Cell hybridization

Tanigawa, G., Jarcho, J.A., Kass, S., Soloman, S.D., Vosberg, H.-P., Seidman, J.G., Seidman, C.E. (1990). A molecular basis for familial hypertrophic cardiomyopathy A cardiac myosin heavy chain hybrid gene. Cell 62,991-998. 

Goate, A.M., Cooper, D.N., Hall, C., Leung, T.K., Solomon, E., Li, L. (1987). Localization of a human heat shock hsp70 gene sequence to chromosome 6 and detection of two other loci by somatic cell hybrid and RFLP analysis. Hum. Gen. 75, 123-128. 

Use of rodent or human somatic cell hybrids Permits assignment of a gene to one specific chromosome but not to a subregion. 

VI3. Vora, S and Francke, U., Assignment of the human gene for liver-type 6-phosphofructokinase isozyme (PFKL) to chromosome 21 by using somatic cell hybrids and monoclonal anti-L antibody. Proc. Natl. Acad. Sci. U.S.A. 78,3738-3742 (1981). 

Volume 327. Applications of Chimeric Genes and Hybrid Proteins (Part B Cell Biology and Physiology)... 

Bennett, K. L., Lalley, P. A., Barth, R. K. and Hastie, N. D. (1982) Mapping the structural genes coding for the major urinary proteins in the mouse combined use of recombinant inbred strains and somatic cell hybrids. Proc. Natl. Acad. Sci. USA 79, 1220-4. 

Human pedigree studies of families with Lesch-Nyhan syndrome (Seegmiller et al., 1976) showed that the HPRT gene was X-linked, and this was confirmed by analysis of human-mouse cell hybrids which had lost most of their human chromosomes (Ricciuiti and Ruddle, 1973). 

The human A locus is located on chromosome 22. As in the case of the human Igh locus, the A genes were first tentatively mapped by studies measuring immunoglobulin gene expression in somatic cell hybrids [91] and the chromosome assignment later confirmed by direct hybridization studies [99]. 

Liu, Z.G., Smith, S.W., McLaughlin, K.A., Schwartz, L.M. and Osborne, B.A. (1994) Apoptotic signals delivered through the T-cell receptor of a T-cell hybrid require the immediate-early gene nur77. Nature 367 281-284. 

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