Gelatin conjugates

Kushibild T, Matsuoka H, Tabata Y (2004) Synthesis and physical characterization of poly(ethylene glycol)-gelatin conjugates. Biomacromolecules 5(l) 202-208... 

Sakai S, Hashimoto I, Kawakami K. Agarose-gelatin conjugate for adherent cell-enclosing capsules. Biotechnol Lett 2007 29 731-735. 

Cirillo G, Kraemer K, Fuessel S, Puoci F, Curcio M, Spizzirri UG, Altimari I, lemma F (2010). The biological activity of a gallic acid-gelatin conjugate. Biomacromoiecuies, 11,3309-3315. 

Direct and indirect competition formats, illustrated in Figure 1, are widely used for both qualitative and quantitative immunoassays. Direct competition immunoassays employ wells, tubes, beads, or membranes (supports) on to which antibodies have been coated and in which proteins such as bovine semm albumin, fish gelatin, or powdered milk have blocked nonspecific binding sites. Solutions containing analyte (test solution) and an analyte-enzyme conjugate are added, and the analyte and antibody are allowed to compete for the antibody binding sites. The system is washed, and enzyme substrates that are converted to a chromophore or fluorophore by the enzyme-tracer complex are added. Subsequent color or fluorescence development is inversely proportionate to the analyte concentration in the test solution. For this assay format, the proper orientation of the coated antibody is important, and anti-host IgG or protein A or protein G has been utilized to orient the antibody. Immunoassays developed for commercial purposes generally employ direct competition formats because of their simplicity and short assay times. The price for simplicity and short assay time is more complex development needed for a satisfactory incorporation of the label into the antibody or analyte without loss of sensitivity. 

Gelatin core with dextran conjugate of drug 15 Lung, after intravenous injection Mitomycin-dextran conjugate... 

Mostly bovine serum albumin (BSA) is used. Gelatin from cold fish, inactivated calf serum, casein hydrolysate, non-fat dry mUk, polyvinylpyrrolidone, or Tween 20 are also suitable. Concentrations from 0.1 to 0.5% (w/v) are sufficient. Casein and mfik are not of first choice if (strept)avidin conjugates are used. 

Receptor-specific antibodies and fluorochrome-conjugated reagents are diluted in PBS-gelatin or PBS-gelatin-Sap (for permeabilized cells) as required. Appropriate antibody dilutions should be determined empirically. Note Cells should not be allowed to dry at any stage in the procedure. 

Chen T, Embtee HD, Wu LQ, Payne GF (2002) In vitro protein-polysaccharide conjugation tyrosinase-catalyzed conjugation of gelatin and chitosan. Biopolymers 64(6) 292-302... 

Dilute rabbit anti-IgG-peroxidase conjugate 1 5000, using 4 JlL of antibody in 20 mL of PBS-gelatin, approx 5 min before use and leave it in ice (i eNote 9). 

Sites on the polystyrene well surface unoccupied by coating conjugate were blocked by adding 200 uL of 0.1% (w/v) gelatin solution in Pi buffer and incubated for 20 min at 4 °C. The plates were emptied and washed as described above. 

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