GDP dissociation inhibitor

GDP-dissociation inhibitors Bind isoprenylated Rab proteins in the GDP-bound form, resulting in a cytoplasmic complex. 

Ataxia telangiectasia mutated (ATM), poly(ADP ribose) polymerase (PARP), DNA-dependent protein kinase, DNA replication factor C, DNA topoisomerase I, DNA fragmentation factor (DFF)45, inhibitor of caspase-activated DNAse (ICAD), lamins A, Bl, and C TRAF-1, Rafl, Ras, GAP, GDP dissociation inhibitor of Rho family GTPases, phospholipase A2, Statl... 

Furthermore, the interaction of prenylated Rah GTPase with REP and GDP dissociation inhibitor (GDI) protein could be studied and analyzed. ... 

Forget, M.A., Desrosiers, R.R., Gingras, D., and Beliveau, R. (2002). Phosphorylation states of Cdc42 and RhoA regulate their interactions with Rho GDP dissociation inhibitor and their extraction from biological membranes. Biochem J 361 243-254. 

Gosser, Y.Q., Nomanbhoy, T.K., Aghazadeh, B., Manor, D., Combs, C, Cerione, R.A., and Rosen, M.K. (1997). C-terminal binding domain of Rho GDP-dissociation inhibitor directs N-terminal inhibitory peptide to GTPases. Nature 387 814-819. 

Pfeffer, S.R., Dirac-Svejstrup, A.B., and Soldati, T. (1995). Rab GDP dissociation inhibitor putting Rab GTPases in the right placs. J Biol Chem 270 17057-17059. 

Willard FS, Siderovski DP. The R6A-1 peptide binds to switch II of Galphai 1 but is not a GDP-dissociation inhibitor. Biochem. Biophys. Res. Commun. 2006 339 1107-1112. 

Enzymatic studies using selectively addressable fluorescent Rab proteins have elucidated the geranylgeranylation process (16). Structural studies on selectively prenylated Rab proteins have elucidated the exact interaction mechanism of Ras proteins with their transporter protein GDP dissociation inhibitor (GDI) and have led to a membrane extraction model for Rab proteins (58, 59). 

Rak A, Pylypenko O, Durek T, Watzke A, Kushnir S, Brunsveld L, Waldmann H, Goody RS, Alexandrov K. Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPTl GTPase. Science 2003 302 646-650. 

Brunsveld L, Watzke A, Durek T, Alexandrov K, Goody RS, Waldmann H. Synthesis of functionalized rab GTPases by a combination of solution- or solid-phase Upopeptide synthesis with expressed protein ligation. Chemistry 2005 11(9) 2756-2772. Rak A, Pylypenko O, Durek T, Watzke A, Kushnir S, Brunsveld L, et al. Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPTl GTPase. Science 2003 302(5645) 646-650. Paulus H. Protein splicing and related forms of protein autoprocessing. Annu. Rev. Biochem. 2000 69(1) 447-496. 

Bourmeyster N, Stasia M-J, Garin J et al. (1992) Copurification of rho protein and the rho-GDP dissociation inhibitor from bovine neutrophil cytosol. Effect of phosphoinosifides on rho ADP-ribosylafion by the C3 exoenzyme of Clostridium botulinum. In Biochemistry 31 12863-9... 

Regazzi R, Kikuchi A, Takai Y ef al. (1992) The small GTP-binding proteins in the cytosol of insulin-secreting cells ore complexed to GDP dissociation inhibitor proteins. In J. Biol. Chem. 267 17512-9... 

In this way, membrane association of prenylated Rho- and Rab-GTPases is modulated by their cognate GDP dissociation inhibitor proteins (Y. An, 2003 A. Rak, 2003). 

Step 2). When a GEF protein (gnanine nucleotide exchange factor) binds to a G-protein, it increases the rate of GTP exchange for a bound GDP, and therefore activates the G-protein (see Fig. 9.13, step 3). GDI proteins (GDP dissociation inhibitor) bind to the GDP-G protein complex and inhibit dissociation of GDP, thereby keeping the G protein inactive. 

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