GAPDH

DASL, cDNA-mediated annealing, selection, extension, and ligation GAPDH, glyceraldehydes-3-phosphate dehydrogenase IBDV, infectious bursal disease virus VEGF, vascular endothelial growth factor. 

Another reason for neglecting perhydroxyl radical is a big difficulty to distinguish it from the much more abundant and more reactive peroxyl radicals. Nonetheless, in several works perhydroxyl radical was considered as a possible initiator of lipid peroxidation (see Chapter 25). It should be noted that at least two biological systems were described where the participation of perhydroxyl radicals seems to be possible. Thus, it has been shown [25,26] that perhydroxyl radical is able to abstract hydrogen atom from NADH (Reaction 6) and the glyceraldehyde-3-phosphate dehydrogenase-NADH (GAPDH-NADH) complex (Reaction 7). 

Found in ocular tissues immunoaffinity purified MW 44 kDa (Ikeda et al., 2003). 0.0032 per GAPDH mRNA in adult human brain by RT-RT-PCR (Nishimura et al, 2003). 

CYP8B1 mRNA/GAPDH mRNA in human brain by RT-RT-PCR (Nishimura et al., 2003). 

CYP8A1 in neurons of bovine, rat and human brain, especially Purkinje cells of cerebellum and cortical neurons, but not glial cells by IHC (Mehl et al., 1999). CYP8A1 mRNA and protein in blood vessels of human brain, pyramidal cells of cortex and hippocampus, and Purkinje cells of cerebellum (Siegle et al., 2000). 0.000161 per GAPDH mRNA in whole adult human brain by RT-RT-PCR (Nishimura et al., 2003). 

Dickey et al. (403) reported a list of tau-reducing compounds identified from an initial in-cell Western screening assay. Drugs resulting in more than 25% reductions in tan levels with less than 10% reductions in GAPDH include aggregation inhibitors (diazaquone, methylene blue) antibiotics (alexidine HCl) antiproliferatives (colchicine, albendazole, chelidonine, rotenone) and steroids (norethindrone) (403). 

Figure 3.22 Array map for GAPDH DNA and 30-mer probe. (From Taylor, S. et al.. Nucleic Acid Res., 31(16), 1-19, 2003. With permission.)...

Key words Transfection, siRNA, GAPDH, Western blotting. Densitometry, pH-responsive peptide. 

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