Galacto-mannanases

Pectinases and galacto-mannanases are the main enzymes used in the coffee industry. Pectinases increase the pulp removal efficiency and galacto-mannanases act as viscosity reducers in production of instant coffee. 

The most characterised D-mannan degrading enzymes are the endo- -mannanases (5). These enzymes act on a range of 1,4-p-D-mannan-type polysaccharides including D-mannan, glucomannans (acetylated and deacetylated), galactomannans and galacto-glucomannans. The extent and... 

This enzyme [EC 3.2.1.78], also known as mannan endo-l,4-j8-mannosidase and endo-l,4-mannanase, catalyzes the random hydrolysis of l,4-j8-D-mannosidic linkages in mannans, galactomannans, glucomannans, and galacto-glucomannans. 

Endo-D-mannanases degrading galacto- and gluco-mannans usually liberate a series of mixed oligosaccharides, in addition to the linear (1 — 4)-/3-D-manno-oligosaccharides. Several of the former oligosaccharides have been isolated, and characterized (usually by methylation), and are described next. 

The phytopathogen Xanthomonas campestris when grown on several polysaccharide substrates, including D-galacto-D-mannan, produced /5-D-mannanases and carboxymethylcellulases which were present as cell-wall- or membrane-... 

Mannans. — D-Galacto-D-mannan dyed with Remazol Brilliant Blue has been used in a simple assay procedure for D-mannanase. The procedure is quantitative, sensitive, and highly specific. 

An endo-p-D-mannanase activity in the endosperm of germinating fenugreek Trigonella foenum-graecum) seeds is required to hydrolyse the storage galacto-mannan. The level of e/iJo-P-D-mannanase activity is controlled in vivo by the aleurone layer. 

A simple assay procedure for j5-D-mannanase enzyme which has been developed employs carob D-galacto-D-mannan dyed with Remazolbrilliant Blue." Additionally, the procedure is quantitative, relatively sensitive, and highly specific for jS-D-mannanase enzyme. It can be readily used for the determination of jS-D-mannanase activity in crude enzyme preparations and column-chromatography eluates. 

The modes of action of jS-o-mannanase enzymes of diverse origin on legume seed D-galacto-D-mannans have been studied." ... 

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