G protein-coupled receptor assay

Guo, T. and Hobbs, D.W. Privileged structure-based combinatorial libraries targeting G protein-coupled receptors. Assay Drug Dev. Tech. 2003, 1, 579-592. 

Bresnick, J.N. et al. 2003. Identification of signal transduction pathways used by orphan G protein-coupled receptors. Assay Drug Dev. Technol. 1, 239-249. 

Williams, C. and A. Sewing. 2005. G protein-coupled receptor assays to measure affinity or efficacy that is the question. Comb. Chem. High Through. Screen. 8, 285-292. 

R.M. Eglen, Functional G protein-coupled receptor assays for primary and secondary screening, Comb. Chem. High Throughput Screen. 2005,8,311-318. 

Christopoulos, A. (2000). Quantification of allosteric interactions at G-protein coupled receptors using radioligand assays. In Current protocol in pharmacology, edited by Enna, S. J., pp. 1.22.21-1.22.40. Wiley and Sons, New York. 

Banks, P., Gosselin, M., and Prystay, L., Impact of a red-shifted dye label for high throughput fluorescence polarization assays of G protein-coupled receptors, /. Biomol. Screen., 5, 329,... 

Table 1 Classification and Description of the Different Targets included in the In vitro Pharmacological BioPrint Profile. The number of assays per each family is detailed. Among the receptors there are 91 G-protein coupled receptors.

Bertrand, L., Parent, S., Caron, M., Legault, M., Joly, E., Angers, S., Bouvier, M., Brown, B., Houle, B., and Menard, L. (2002). The BRET2/arrestin assay in stable recombinant cells A platform to screen for compounds that interact with G protein-coupled receptors (GPCRs)./ Recept. Signal Transduct. Res. 22, 533-541. 

MH Pausch. G protein-coupled receptors in Saccharomyces cerevisiae. high-throughput screening assays for drug discovery. Trends Biotechnol 15 487-494, 1997. 

G-protein coupled receptor (GPCR)—ligand binding assays... 

Protein assays using ordered arrays emerged in the late 90s [13]. Recently Lahiri et al. introduced a technique which enables the fabrication of microarrays for membrane proteins [14]. Printed lipid microspots on y-aminopropylsilane slides have high mechanical stability, independent of whether the lipid is in the gel-or fluid-phase. They immobilized G-protein coupled receptors and studied their interaction with a set of inhibitors. 

ASSAY DEVELOPMENT FOR G PROTEIN-COUPLED RECEPTORS 4.2.1 Biology of G Protein-Coupled Receptors... 

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