Furazolidone analysis

Separations were performed on either silica- or amino-bonded stationary phase columns, using carbon dioxide with various modifiers as mobile phases. Each column exhibited distinctly different selectivities to the examined sulfonamides, the amino-bonded column being much more sensitive to modifier variations. In a continuation of the sulfonamide study, packed-column SFC was further evaluated for possible application to the analysis of furazolidone, chloramphenicol, and lincomycin residues (82). Separation was effected on an amino-bonded stationary phase using carbon dioxide with methanol modifier as the mobile phase, whereas detection was accomplished by MS. 

A multiresidue preparation technique—MSPD—has also been applied to the analysis of CAP residues in meat samples. Two fractions were collected by elution with methylene chloride and ethyl acetate. No additional purification was necessary. Diode assay detection and fluorescence detectors were recommended for the multiresidue analysis of sulfonamides, benzimidazoles, nicarbazin, furazolidone, and CAP. The percentage recoveries and linearity of the method were evaluated. The method was linear from 50 to 250 /tg/kg of CAP. Not only do the authors recommend the MSPD multiresidue procedure for HPLC analysis, but it could be associated with several detection modes, such as immuno- or receptor assays. The MSPD technique represents a new approach in the field of biological-matrix extraction and provides a great possibility for the analysis of a wide range of compounds (20). 

V Hormazdbal, M Yndestad. Simple and rapid method of analysis for furazolidone in meat tissues by high-performance liquid chromatography. J Liq Chromatogr 18 1871-1877, 1995. 

E Horne, A Cadogan, M Okeeffe, LAP Hoogenboom. Analysis of protein-bound metabolites of furazolidone and furaltadone in pig-liver by high-performance liquid chromatography and liquid chromatography-mass spectrometry. Analyst 121 1463-1468, 1996. 

The separations of nitrofurans have been carried out, in most instances, with a reversed phase (RP) column. As summarized in Table 1, Cig columns were selected as the analytical column for LC in most reports however, the use of a Cg, CN, and cyanopropyl-modified silica analytical column has also been described. The parent compounds, as well as their metabolites, are satisfactorily separated by Cig columns with commonly used mobile phases, such as mixtures of acetonitrile with phosphate buffer, acetate buffer, or water. The mobile phase elution for HPLC analysis can be isocratic or as a gradient system. Acetonitrile-0.1 M aqueous solution of sodium perchlorate (28 72), with 0.5% glacial acetic acid, was reported by Galeano Diaz " as an optimum mobile phase for the separation of the three nitrofuran derivatives nitrofurantoin, furazolidone, and furaltadone in milk. Lin et al." used an acetonitrile gradient with an initial hold time of 1 min at 0% acetonitrile. 

UV detection and diode-array detection (DAD) have been widely used, coupled to HPLC, for the analysis of the parent compounds of nitrofurans. The detection wavelengths of nitrofiirans have been set at 368 nm," 365 nm, " 360 nm," 270 nm, or at 254 nm. " An HPLC method, with electrochemical detection, has also been described for the monitoring of furazolidone and furaltadone hydrochloride in chickoi tissues and eggs. " ... 

Three nitrofuran derivatives, nitrofurantoin, furazolidone and furaltadone (Figure 7.7), have been measured in cow s milk after deproteinisation with TCA and SPE (ODS-modified silica). The analysis was performed using an ODS-modifed silica column with 0.5% (v/v) acetic acid in acetonitrile-aq. sodium... 

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