Fungi colonial

Summation of mesophilic bacteria, fungi, and thermophilic bacteria. CPU = colony-forming units. 

The colony characteristics and microscopic morphology used for the identification of dematiaceous fungi are based upon cultures that are approximately 2 weeks old and have been grown at 25 to 30°C on a medium such as potato dextrose agar or cornmeal agar. These media... 

After the six-month field test, the Prototype Unit was disassembled and the component parts were individually swabbed with sterilized cotton wools (4 cm2). Each samples were stored in 1 ml sterilized distilled water and 50 pi of samples were transferred to TSA and MEA plates. The TSA plates were incubated at 37 °C for 24 h and bacterial colonies were counted. The number of fungal colonies was determined from the MEA plates after incubating at 30 °C for 5 days. The results of the test are shown in Table 12.9-7. The results show that bacteria and fungi thrived near the air intake where they deposited on the grills, panel and around the intake slots. However once the microorganisms are drawn through the catalyst-coated desiccant wheel, the number of viable... 

Protists Eukaryotic microorganisms that are neither animal, fungi, plant, or archaean. Unicellular forms include the amoeboid protozoans and algae, such as the foraminferans and radiolarians, and dinoflagellates and diatoms, respectively. Some algae are either multicellular or colonial, such as the red algae and freshwater Volvox, respectively. 

We have studied CNT influence on growth rate and proliferation of some cellular colonies [29]. Interesting results were obtained in case of bread-making yeast-like fungi Saccharomyces cerevisiae (strain 608) and hamster kidney cells. Introduction of small amounts of CNT ( 3 pg/mL) in fungal suspensions led to 2-fold increase in Saccharomyces cerevisiae colonies number compared to the control, after 48 h of incubation at 30°C (Fig. 2.2). Similar results were obtained for colonies of hamster kidney cells. Presence of CNT activated cell proliferation and increased cell growth rate by 1.5 times. 

An action level in microbiological environmental monitoring is that level of microorganisms that, when exceeded, requires immediate follow-up and, if necessary, corrective action. The evaluation does not depend only on the number of colonies but also on the types of microbes isolated and the suspected hazard from them, e.g.. Gram-negative bacteria, anaerobic bacteria, fungi, or organisms not normally isolated. 

Surface growth (colonies oil plates) Microscopy (cell-shapes) Yeasts (yeast-like fungi)... 

Fig. 9.3. Recovery of fungi from pPVC on plasticizer agar. Hydrolysis of the plasticizer can be seen as a zone of clearing around some colonies.

Fig. 10.2. Relationship between values of (a) pH of agar and zinc phosphate (= 5mM) solubilization measured as clear halo diameters in the agar around colonies of mycorrhizal fungi (see Fomina et al., 2005a) (b) pH of fungal culture filtrates and pyromorphite solubilization measured as the ratio (%) of free metal concentration in the liquid phase to that in the initial solid phase (initial (pyromorphite) = 0.5 mM) (c) as above but excluding oxalate over-excreting Beauveria caledonica (see Fomina et al., 2004).

Many fungi can precipitate reduced forms of metals and metalloids (e.g. elemental silver, selenium, tellurium) within and around fungal cells (Gadd, 2004). The reductive ability of fungi is manifest by the appearance of black coloration of fungal colonies precipitating elemental silver or tellurium, and a red coloration for precipitation of elemental selenium (Gharieb et al., 1999). 

Gorbushina, A. A., Whitehead, K., Dornieden, T. et al. (2003). Black fungal colonies as units of survival hyphal mycosporines synthesized by rock-dwelling microcolonial fungi. Canadian Journal of Botany, 81, 131-8. 

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