Proteolytic Activity, Fungal

Lipase (Microbial) Activity for Medium- and Long-Chain Fatty Acids, (S3)105 Lysozyme Activity, (S3)106 Maltogenic Amylase Activity, 804 Milk-Clotting Activity, 805 Pancreatin Activity, 805 Pepsin Activity, 807 Phospholipase A2 Activity, 808 Phytase Activity, 808 Plant Proteolytic Activity, 810 Proteolytic Activity, Bacterial (PC), 811 Proteolytic Activity, Fungal (HUT), 812 Proteolytic Activity, Fungal (SAP), 813 Pullulanase Activity, 814 Trypsin Activity, 814 Enzyme Assays, 786 Enzyme-Hydrolyzed (Source) Protein,... 

Some of the basic information on stabilizing sulfhydryl enzymes, has been responsible for their commercialization. Without the judicious use of reducing compounds throughout the processing of the papaya latex, it would not have been possible to maximize the proteolytic activity of commercial papain preparations. Examples of other studies of enzymes which have contributed to commercialization are the determination of calcium ion as a requirement for amylase stability at high temperature, the difference in properties of catalases derived from bacterial, fungal, or... 

Rhozyme [Rohm Haas]. TM for enzyme concentrate with diastatic or proteolytic activity. Buff-colored powders or liquids of fungal or bacterial origin that hydrolyze and solublize proteins and starches. 

Aspartic proteases form a group of proteolytic enzymes that catalyze peptide bond cleavage by acid-base catalysis and activation of a water molecule for nucleophilic attack on the amide carbon. Crystal structures of mammalian and fungal enzymes are known. In pepsin, the best studied aspartic protease, catalysis proceeds by water activation and leaving group protonation. Both involve an aspartate, which explains the low pH optimum of around 4. The two aspartic acid residues are situated around a hydrophobic deft that can accommodate seven amino acids (the S4-S3 subsites). The active site is covered by a flexible flap, which contributes to SI subsite specificity. 

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