Fungal Expression Systems

New transgenic production systems are emerging that possess the potential to become industrially successful (e.g., transgenic moss, lemna, fungal or yeast expression systems, transgenic animals and... 

Furthermore, Asp77 is shown to be the binding site to Pi Lys in a substrate by crystallographic study of penicillopepsin [25, 26] and site-directed mutagenesis studies of rhizopuspepsin (31). Using the S. cerevisiae expression system, Tyr77 on the flap of mucorpepsin (32) has been shown to enhance catalytic efficiency of this fungal aspartic proteinase. 

All expression systems, bacterial, fungal, insect cells, and mammalian cells, are useful tools in molecular pharmacology in different situations (Abbot, 1991 Hodgson, 1992). For many cell-surface molecules and receptors, mammalian cells are the best choice for the functional study of isolated proteins. Further, the use of a mammalian cell line that does not normally express the protein under study is helpful because... 

The production of fuU-sized antibodies is restricted to mammahan cell systems that possess the correct production and glycosylation machinery (see Part IV, Chapter 1). Antibody fragments, on the other hand, can also be expressed at much lower cost in microbial expression systems, such as bacterial, yeast, or fungal fermentation [49, 50]. For the high-level expression of antibody fragments, E. coli fermentation provides a well-established tech-... 

The P. past oris expression system produced high yields of secreted protein. The recombinant protein represents the majority of the protein supernatant without further purification. The E. coli expression system was found to be unsuitable for expression of soluble and active ferulic acid esterase from a fungal source. 

MCO enzymes for EFC technology have eome Irom a wide variety of plant, fungal, bacterial, and even human sources. To best deseribe and understand these expression systems, the application of several published expression systems will be detailed. The majority of MCOs used for cathodie redox reaetions in EFC studies have been laceases, AOx, and BOx their heterologous expression wiU be outlined in the next seetion, with a brief summary of other MCOs sueh as eemloplasmin (CP) and bacterial MCOs. 

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