Fundamental of Enzyme Kinetics

Basic approaches to solve enzyme kinetics follow  

System A simplified reaction involving one substrate (uni-substrate enzyme catalysis) for the forward direction as shown by  

Condition Initial velocities (v) are generally measured at a very low enzyme concentration (i.e. 10 -10 °M) compared with substrate concentrations (A), which are usually greater than 10 M. Thus 

Initial velocity expression Identification of the rate-determining step and description of the initial velocity expression  

Kinetic treatments Enzyme kinetic data are treated according to two assumptions  

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Copeland, R. A., and Anderson, P. S. (2001) in Textbook of Drug Design and Discovery, 3rd ed., P. Krogsgaard-Larsen, T. Liljefors, and U. Madsen, eds., Taylor and Francis, New York, pp. 328—363. Cornish-Bowden, A. (1995), Fundamentals of Enzyme Kinetics, Portland Press, London. 

Cornish-Bowden, A. Fundamentals of Enzyme Kinetics , Portland Press London, 1995. 

Cornish-Bowden, A. 1999. Fundamentals of enzyme kinetics, Revised Edition. London Portland Press. 

Comish-Bowden, A. (1995) Fundamentals of Enzyme Kinetics. London Portland Press. Fersht, A. (1985) Enzyme Stmcture and Mechanism. Reading W.H.Freeman. 

Cornish-Bowden, A. (1979) Fundamentals of Enzyme Kinetics. Butterworths, London. Costello, S.M., Felix, R.T. and Giese, R.W. (1979) Clin Chem. 25, 1572. 

Cornish-Bowden A (1995) Fundamentals of enzyme kinetics, 2nd edn. Portland Press, London, 344 pp... 

Properties. — A book entitled Fundamentals of Enzyme Kinetics covers all of the enzyme kinetics likely to be required by a student, and provides sufficient additional material to serve as a useful introduction to the subject for research workers coming to biochemistry from other disciplines. The practical aspects of the subject are emphasized and the author discusses methods of purifying... 

Figure 19.9 Interpretation of primary kinetic isotope effects. The bond energy a is shown as a function of R, the bond length. Most molecules are in their lowest vibrational energy state at ordinary temperatures. This state is lower by about 4.8 kj mol in a C-D bond than in a C-H bond. So the C-D bond requires about 4.8 kJ mol more energy to reach the same transition state. Source A Cornish-Bowden, Fundamentals of Enzyme Kinetics, Portland Press, London, 1995. 

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