Fructose-1,6-bisphosphatase gene

Johnson, K.A., Chen, L., Yang, H., Roberts, M.F., and Stec, B., 2001, Crystal structure and catalytic mechanism of the MJ0109 gene product A bifunctional enzyme with inositol monophosphatase and fructose 1,6-bisphosphatase activities. Biochemistry 40 618-630. 

Shin DJ and McGrane MM (1997) Vitamin A regulates genes involved in hepatic gluconeogenesis in mice phosphoenolpyruvate carboxykinase, fructose-l,6-bis-phosphatase and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase./owmaZ of Nutrition 127, 1274-8. 

V.F., and Sahm, H. (2003) Fructose-1,6-bisphosphatase from Corynehacterium glutamicum expression and deletion of the fbp gene and biochemical characterization of the enzyme. Arch. Microbiol, 180 (4), 285-292. 

We have isolated and sequenced cDNAs for wheat phosphoribulokinase (PRK), for chloroplast fructose-1, 6-bisphosphatase (FBP) and for both the chloroplast and cj osolic versions of phosphoglycerate kinase (PGK). We are at present sequencing a putative cDNA clone for sedoheptulose-l,7-bisphosphatase (SBP) and isolating the coding sequence of cytosolic FBP. For the sake of comparison, we have also sequenced the E coU gene for FBP (3). 

From what has been said it is apparent that it is the different enzyme patterns which arise firom the tissue-specific expression of genes that mainly account for the varying metabolic capabilities of different tissue. Thus, for example, the enzymes fructose 1,6-bisphosphatase and glucose 6-phosphatase occur only in liver and kidney and these are the only tissues capable of gluconeogenesis. Similarly, carbamoyl phosphate synthase, which is involved in the synthesis of urea and is ammonia dependent, occurs in the mitochondria of liver but not of other tissues. At the same time, the enzymic profile of tissues can vary to some extent in response to external factors such as diet. 

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