Fructooligosaccharide synthesis

Efficient Fructooligosaccharide Synthesis with a Fructosyltransferase from Aspergillus aculeatus... 

Both fructooligosaccharide synthesis and sucrose hydrolysis are catalyzed by most of the fructosyltransferases and 3-fmctofuranosidases (invertases) in the presence of sucrose. The transferase hydrolase raho, which determines the maximum yield of fructooligosaccharide, depends basically on two parameters the concentra-hon of sucrose and the intrinsic enzyme properties, that is its ability to bind the nucleophile (to which a fructose is transferred) and to exclude H2O from the acceptor binding site [11]. 

I 77 Efficient Fructooligosaccharide Synthesis with a Fructosyitransferase from Aspergillus aculeatus Table 11.3 Main properties of the Sepabeads EC-EP carriers. 

For the above reasons, the effect of pH and ionic shength on the immobihzation of the fructosyitransferase from A. aculeatus on Sepabeads EC-EP was studied. Although this enzyme constitutes only a minor percentage (0.4% w/w) of the total proteins present in Pectinex Ulha SP-L, the commercial preparation was used directly (without enzyme purification) in order to develop a simple process that could be easily scaleable in the industry. It was considered that, although the other proteins present in Pectinex Ulha SP-L could also be immobilized in Sepabeads EC-EP, they would not interfere in fructooligosaccharide synthesis experiments. 

Sugar Beet Syrup and Molasses as Low-cost Feedstock for Fructooligosaccharide Synthesis... 

Beine R, Moraru R, Nimtz M, Na amnieh S, Pawlowski A, Buchholz K, Seibel J (2008) Synthesis of novel fructooligosaccharides by substrate and enzyme engineering. J Biotechnol 138 33—41... 

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