Freeze-thaw procedure

A quantitative reconstitution approach was used to gain information as to the subunit composition of the -ATPase molecule [25]. Proteoliposomes prepared from asolectin and purified, radiolabeled ATPase molecules obtained by a freeze-thaw procedure similar to that of Dufour et al. [34] were shown to catalyze ATP hydrolysis-driven proton translocation, as indicated by the extensive quenching of aminochloromethoxyacridine fluorescence that occurs upon the addition of MgATP to the proteoliposomes, and the reversal of this quenching induced by... 

In conclusion, lipid vesicles that are fully equilibrated (freeze-thaw procedure) may be stabilised by both translational as well as undulational entropy. 

The injeetion method and reverse phase dialysis are additional procedures for the formation of SUV and LUV. Freeze-thaw procedures allow drug loading of the liposomes and offer an evaluation of the stability of the vesieular dispersion. For further reading. Refs. 7 and 8 are recommended. 

Stability Pass 4+ weeks 9 50C, 5 cycles of freeze/thaw Procedure ... 

X-ray photoelectron (ESCA) spectra of carbenium ions have also been obtained in frozen superacid solutions, generally in SbF5-S02 solution or as isolated salts. Sulfur dioxide was subsequently removed by the usual freeze-thaw procedure. A thin layer of the viscous SbF5 solution was deposited on the precooled sample holder, in a dry nitrogen... 

Standard practice for performing accelerated outdoor weathering of factory-coated embossed hardboard using concentrated natural sunlight and a soak-freeze-thaw procedure. 

D5722—08 Standard Practice for Performing Accelerated Outdoor Weathering of Factory-Coated Embossed Hardboard Using Concentrated Natural Sunlight and a Soak-Freeze-Thaw Procedure... 

Takamura A, Ishii T, Hidaka H (1992) Drug release from poly(vinyl alcohol) gel prepared by freeze-thaw procedure. J Control Release 20 21... 

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