FlyBase Project

Instructions for citing FlyBase are given in Reference Manual I The FlyBase Project. At the time of writing, the correct citation is ... 

FlyBase (1999) The FlyBase database of the Drosophila genome projects and community literature. Nucleic Acids Research 27, 85-88. 

The inverse PCR protocol given below was developed to isolate genomic DNA sequences immediately adjacent to the insertion of large numbers of P-elements as part of the gene-disruption project of the BDGP It has been successfully applied to various P-ele-ments including the P PZ (Mlodzik and Hiromi 1992), P lacW (Bier et al 1989), and P EP (Rorth 1996) elements. Sequences and maps of these P-elements can be found on Flybase at ... 

The Clones section of FlyBase contains lists of genomic and cDNA clones, chiefly from the BDGP and EDGP, but complete reports about these clones can be obtained only from the genome project Web sites, most easily using the BFD search tools. 

The EST (expressed sequence tag) section of FlyBase links to data from two different BDGP projects the main BDGP/HHMI project sequencing the ends of full-length cDNAs and a small-scale project analyzing membrane-associated cDNAs (the CK library). 

The FlyBase Consortium 1999. The FlyBase Database of the Drosophila Genome Projects and Community Literature. Nucleic Acids Res. 27 85-88. 

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