Fluorescent microbe detection

The performance of a biotreatment system ultimately depends on optimization of the activity of microbes and the ability to control the process parameters of the treatment system [157]. In this respect, the ability to monitor gene copy numbers and gene expression is highly useful for real time optimization of the efficiency of a biotreatment system. Advanced molecular techniques as well as low cost methods (e.g., antibody detection of enzymes based on color reaction strips fluorescence i.e., GFP marked organisms with UV light detection) can also be applied to monitor the microbial community structure, persistence of the added bacteria, and their interactions with indigenous populations. 

Lemarchand K, Parthuisot N, Catala P, Lebaron P (2001) Comparative assessment of epifluores-cence microscopy, flow cytometry and solid phase cytometry in the enumeration of specific bacteria in water. Aquat Microb Ecol 25 301-309 Lepeuple AS, Delabre K, GUouppe S, IntertagUa L, de Roubin MR (2003) Laser scanning detection of FISH-labelled Escherichia coli from water samples. Water Sd Technol 47 123-129 Lisle JT, Hamilton MA, Willse AR, McFeters GA (2004) Comparison of fluorescence microscopy and sohd-phase cytometry methods for counting bacteria in water. Appl Environ Microbiol 70 5343-5348... 

The previously described device under development at Utah State University for detection of total pathogenic microbes (including spores) is an example of a ligand-hased probe with fluorescence-based transduction. A handheld unit simply using fluorescence to determine total viable... 

Oppedahl, A. M. Lasky, S. J. Baker, D. D. Buttry, D. A. Steger, A. M. Simultaneous rapid detection of more than one microbe class using labeling agents including fluorescent labels. U.S. Pat. Appl. Publ. US 2008261229,2008 Chem. Abstr. 2008,149,465856. 

Goodridge L, Chen JR, Griffiths M (1999) Development and characterization of a fluorescent bacteriophage assay for detection of Escherichia coli 0157 H7. Appl Environ Microb 65 1397-1404... 

---