Fluorescence resonance energy applications

Oswald B, Gruber M, Bohmer M, Lehmann F, Probst M, Wolfbeis OS (2001) Novel diode laser-compatible fluorophores and their application to single molecule detection, protein labeling and fluorescence resonance energy transfer immunoassay. Photochem Photobiol 74 237-245... 

Szolldsi, J., Damjanovich, S. and Matyus, L. (1998). Application of fluorescence resonance energy transfer in the clinical laboratory Routine and research. Cytometry 34, 159-79. 

Jovin, T. and Arndt-Jovin, D. (1989). FRET microscopy Digital imaging of fluorescence resonance energy transfer. Application in cell biology. In Cell Structure and Function by Microspectrofluorometry (Kohen, E., JG, H. and Ploem, J., eds.). Academic Press, London, pp. 99-117. 

Didenko, V. V. (2001). DNA probes using fluorescence resonance energy transfer (FRET) Designs and applications. Biotechniques 31,1106-1116. 

Blagoi, G., Rosenzweig, N. and Rosenzweig, Z. (2005). Design, synthesis, and application of particle-based fluorescence resonance energy transfer sensors for carbohydrates and glycoproteins. Anal. Chem. 77, 393-399. 

Ecker, R. C., de Martin, R., Steiner, G. E. and Schmid, J. A. (2004). Application of spectral imaging microscopy in cytomics and fluorescence resonance energy transfer (FRET) analysis. Cytometry A 59, 172-81. 

The upgrade of a frequency-domain fluorescence lifetime imaging microscope (FLIM) to a prismless objective-based total internal reflection-FLIM (TIR-FLIM) system is described. By off-axis coupling of the intensity-modulated laser from a fiber and using a high numerical aperture oil objective, TIR-FLIM can be readily achieved. The usefulness of the technique is demonstrated by a fluorescence resonance energy transfer study of Annexin A4 relocation and two-dimensional crystal formation near the plasma membrane of cultured mammalian cells. Possible future applications and comparison to other techniques are discussed. 

Clapp AR et al (2007) Two-Photon excitation of quantum-dot-based fluorescence resonance energy transfer and its applications. Adv Mater 19 1921-1926... 

U. Kubitscheck, M. Kircheis, R. Schweitzer-Stenner, W. Dreybrodt, T. M. Jovin, and J. Pecht, Fluorescence resonance energy transfer on single living cells. Application to binding of monovalent haptens to cell-bound immunoglobulin E, Biophys. J. 60, 307-318 (1991). 

New boxed applications include an arsenic biosensor (Chapter 0). microcantilevers to measure attograms of mass (Chapter 2), molecular wire (Chapter 14), a fluorescence resonance energy transfer biosensor (Chapter 19), cavity ring-down spectroscopy for ulcer diagnosis (Chapter 20), and environmental mercury analysis by atomic fluorescence (Chapter 21). 

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