Fluorescence polarization spectroscopy

Fisz, J. J. (2007). Fluorescence polarization spectroscopy at combined high-aperture excitation and detection Application to one-photon-excitation fluorescence microscopy. J. Phys. Chem. A 111, 8606-21. 

Tachi T, Kaji N, Tokeshi M, Baba Y (2009) Microchip-based homogeneous immunoassay using fluorescence polarization spectroscopy. Lab Chip 9 966-971... 

Heparin has been fluorescently labeUed in a manner that does not alter the functional properties of the polysaccharide. The labelled polysaccharide has been used in conjunction with fluorescence polarization spectroscopy to monitor the binding of heparin to the L-serine proteases thrombin, factor XIa, factor Xa, and plasmin. The stoicheiometry and dissociation constants of the interactions have been measured. The kinetics of inactivation of the four proteases by anti-thrombin as a function of heparin concentration have also been measured. Evidence shows that the direct binding of heparin to anti-thrombin is probably responsible for the polysaccharide-dependent acceleration of hemostatic enzyme-inhibitor reactions. 

Nanosecond fluorescence polarization spectroscopy is a technique well suited for the analysis of the conformation and dynamic properties of macromolecules (1,2). Fluorescence polarization measurements have until recently been mainly carried out under steady state conditions, but in recent years the time dependence of fluorescence polarization has been successfully applied to the study of size, shape and segmental flexibility of macromolecules (3,4) with the added advantage that complex rotational motions can often be explicitly resolved on the time axis (5). 

D.B. Tata, M. Foresti, J. Cardero, P. Thomachefsky, M.A. Alfano, R.R. Alfano Fluorescence polarization spectroscopy and time-resolved fluorescence kinetics of native cancerous and normal rat kidney tissues. Biophys. J. 50, 463 (1986)... 

Steady-State Fluorescence Polarization Spectroscopy as a Tool to Determine Microviscosity and Structural Order in Lipid Systems... 

The next section presents examples of the many applications of fluorescence polarization spectroscopy in food research, some of which are presently being developed in my laboratory. 

Some practical applications of steady-state fluorescence polarization spectroscopy in food-related research are presented in this section. The nature of the... 

In this chapter, a general introduction to the theory and application of fluorescence polarization spectroscopy has been presented. This technique opens up the interesting possibility of studying the hydrodynamic properties of a fluid at the molecular level. With the growing importance of knowledge of the structure of lipid liquid phases (e.g., liquid crystalline phases), this technique may find more widespread application in fats and oils research. 

Murakami, A. Nakaura, M. Nakatsuji, Y. Nagahara, S. Tran, C. Q. Makino, K. Fluorescent-labeled oligonucleotide probes detection of hybrid formation in solution by fluorescence polarization spectroscopy. 

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