Oligonucleotide probe fluorescent-labeled

A useful approach to monitor microbial populations in the biotreatment of hazardous wastes involves the detection of specific sequences of nucleic acids by hybridization with complementary oligonucleotide probes. Radioactive labels, fluorescent labels, and other kinds of labels are attached to the probes to increase sensitivity and simplicity of the hybridization... 

Fuchs, B. M. Wallner, G. Beisker, W. Schwippl, I. Ludwig, W. Amann, R. Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes. Appl. Environ. Microbiol. 1998, 64, 4973 1982. 

Oligonucleotide probes may be labeled with small fluorescent molecules for detection of hybridization by luminescence. Fluorescent probes are widely used in assay systems involving bio-specific interactions (Chapter 9). Receptors for ligands may be localized in tissues or cells by modification of the ligand with the appropriate fluorophore. Targeted molecules may be... 

The sensitivities of the label-based approach depend mainly on the specific activity of the labels linked to the oligonucleotide probe. Radioisotopic [48], fluorescent [50] and enzymatic [38,40,47] labels have been commonly used. Besides the above labels, NPs have attractive properties to act as DNA tags [18,51]. The fact that NPs present an excellent biocompatibility with biomolecules and display unique structural, electronic, magnetic, optical and catalytic properties have made them a very attractive material to be used as label [52,53]. 

Hybridisation can thus be used to produce probes, for example, for the detection of oligonucleotides in chick embryo tissue or of Epstein-Barr viruses (glandular fever) in transplant recipients - by detection of specific RNA strands. DNA dendrimers and corresponding detection devices have meanwhile become commercially available (Genisphere company). Fluorescence-labelled polynucleotide dendrimers have also been used for signal intensification in DNA microchip technology [59]. 

Thiazole orange derivatives have also been covalently attached to oligonucleotides to generate a fluorescently labelled probe oligonucleotide capable... 

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