Fluorescence Imaging Methods

Waters JC. Live-cell fluorescence imaging. Methods Cell.BioI. 2007 81 115-140. 

A series of fatty acids (FAs) were conjugated with polyethylenimine (PEI 25 kDa) (Figure 20.2). A near-infrared fluorescence probe, IR820, was used to study in vivo and ex vivo the brain targeting ability of these fatty acid-PEI conjugates (FA-PEIs). Brain uptake of FA-PEI(25 kDa)/rhodamine-6-iso-thiocyanate (RITC)-labeled DNA nanoparticles was investigated via a fluorescence imaging method. 

Harashima, H. Akita, H. Development of the fluorescent imaging method for quantifying nucleic acid incorporated into target organelle. PCT Int. Appl. WO 2004108962, 2004 Chem. Abstr. 2004,142, i iCll. 

A. Pifferi, K. Svanberg, S. Svanberg, P. Taroni, G. Veilentini, I. Wang Preliminary evaluation of two fluorescence imaging methods for the detection and the delineation of basal cell carcinomas of the skin. Lasers Surg. Med. 26, 76 (2000)... 

FIGURE 14.7 Quantitative fluorescence imaging method for the measurement of solute permeability in a rat pial microvessel. The images were collected during the in vivo experiments and the fluorescence intensity was analyzed off-line. When the fluorescence labeled test solute was injected into the carotid artery, the pial microvessel lumen filled with fluorescent solute (red frame in b), producing AJ . With continued perfusion, the measured fluorescence intensity increased indicating further transport of the solute out of the microvessel and into the surrounding tissue. The initial solute flux into the tissue was measured from the slope (dJ/dt)o (a). The solute permeability P was calculated by P= 1/Afo (dl/dt) rl2. Here, r is the microvessel radius. The scale bar in (b) is 50 Um. (Redrawn from Yuan W et al. 2009. Microvasc Res. 77 166.)... 

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