Organelle targeting

Rosania GR, Lee JW, Ding L, Yoon HS, Chang YT (2003) Combinatorial approach to organelle-targeted fluorescent library based on the styryl scaffold. J Am Chem Soc 125 1130-1131... 

Rizzuto, R., Brini, M., De Giorgi, F., Rossi, R., Heim, R., Tsien, R. Y. and Pozzan, T. (1996). Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo. Curr. Biol. 6, 183-8. 

Torchilin VP (2006) Recent approaches to intracellular delivery of drugs and DNA and organelle targeting. Ann Rev Biomed Eng 8 343-375... 

Rosania et al. [19] have shown this straightforward preparation to be advantageous for the transposition to a diversity-oriented, combinatorial approach to an organelle-targeted fluorescent library. Therefore, the condensation of 9 and 10 (Fig. 5.4) with pyrrolidine as a catalyst was performed in 96-well plates and the dehydration reaction was accelerated by microwave irradiation for 5 min to give 10-90% conversion. The resulting library compounds were analyzed using an LC-MS system with diode-array and fluorescence detectors and a fluorescence plate-reader to determine the absorption and emission maxima and the emission colors. 

Order, cellular delivery system or component Cell-specific homing sequence Organelle targeting sequence Signal sequence Cell-penetrating vector Delivery vehicle Order, immunological peptide Antigen... 

BoddapatiSV etal (2008) Organelle-targeted nanocarriers Specific delivery of liposomal ceramide to mitochondria enhances its cytotoxicity in vitro and in vivo. Nano Lett 8 2559-2563... 

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