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Fluorescence-based hybridization

Patsenker LD, Povrozin YA, Sidorov VI, Tatarets AL, Terpetschnig EA (2009) Fluorescence lifetime based hybridization assay using the new long-wavelength fluorescent label Seta-670. In 24th International conference on photochemistry (ICP 2009). Book of Abstracts, p 430... [Pg.101]

Fluorescent mesoporous hybrid materials based on GFP adsorbed into SBA-15... [Pg.11]

Enzymes useful for detection purposes in ELISA techniques (Chapter 26) also can be employed in the creation of highly sensitive DNA probes for hybridization assays. The attached enzyme molecule provides detectability for the oligonucleotide through turnover of substrates that can produce chromogenic or fluorescent products. Enzyme-based hybridization assays are perhaps the most common method of nonradioactive detection used in nucleic acid chemistry today. The sensitivity of enzyme-labeled probes can approach or equal that of radiolabeled nucleic acids, thus eliminating the need for radioactivity in most assay systems. [Pg.992]

As well as fluorescence-based assays, artificial membranes on the surface of biosensors offered new tools for the study of lipopeptides. In a commercial BIA-core system [231] a hydrophobic SPR sensor with an alkane thiol surface was incubated with vesicles of defined size distribution generating a hybrid membrane by fusion of the lipid vesicles with the alkane thiol layer [232]. If the vesicles contain biotinylated lipopeptides their membrane anchoring can be analyzed by incubation with streptavidine. Accordingly, experiments with lipopeptides representing the C-terminal sequence of N-Ras show clear differences between single and double hydrophobic modified peptides in their ability to persist in the lipid layer [233]. [Pg.107]

Another class of readout measures RNA expression levels, with the three most common methods being chip-based hybridization/fluorescence techniques, realtime polymerase chain reaction (RT-PCR) and quantitative nuclease protection assays (QNPA) [48, 49]. Chip-based methods are widely used for whole-genome scans (discussed in more detail below), but have a disadvantage that they are relatively expensive and so are not really high throughput. The quantitative reproducibility and dynamic range of these chip-based methods are also lower than for the other RNA readout techniques. RT-PCR is a more quantitative technique for measuring transcript levels, and is typically run for up to 40 transcripts at a time. QNPA is another... [Pg.29]

Fluorescence-Based Nucleic Acid Hybridization Assays. 245... [Pg.227]

Schutz E, von Ahsen N, Oellerich M. Genotyping of eight thiopurine methyltransferase mutations three-color multiplexing, two-color/shared anchor, and fluorescence-quenching hybridization probe assays based on thermodynamic nearest-neighbor probe design. Clin Chem 2000 46 1728-1737. [Pg.460]

Schultz E, Galland R, Du Bouetiez D et al (2008) A novel fluorescence-based array biosensor principle and application to DNA hybridization assays. Biosens Bioelectron 23 987-994... [Pg.17]

Moore P, Clayton J (2003) To affinity and beyond. Nature 426 725-731 Budach W, Abel AP, Bruno AP et al (1999) Planar waveguides as high performance sensing platforms for fluorescence-based multiplexed oligonucleotide hybridization assays. Anal Chem 71 3347-3355... [Pg.17]

Letsinger R. Elghanian G. Viswanadham, a fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles. Analytical Chemistry 2000, 72, 5535-5541. [Pg.641]

Using DNA-AuNP probes compared to standard fluorescence-based probes is two orders of magnimde more sensitive when combined with stringency washes at an elevated temperamre. A stringent condition is one under which the majority of the perfectly complementary sequences remain hybridized, while nonspecific and mismatched sequences are washed away. In addition, it was demonstrated that single base imperfections could be detected with increased selectivity (factor of 4) and sensitivity [10,000 times, limit of detection (LOD) = 50 fM] when compared to the corresponding fluorophore labeling methods (Fig. 12.10). [Pg.418]


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Fluorescence-based

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