Fluidity mitochondrial

Carafoli, E., Santella, L., Branca, D., and Brini, M., 2001, Generation, control, and processing of cellular calcium signals, Crit. Rev. Biochem. Mol. Biol. 36, pp. 107-260 Chalmers, S. and Nicholls, D. G., 2003, The Relationship between Free and Total Calcium Concentrations in the Matrix of Liver and Brain Mitochondria, J. Biol. Chem. 278, p. 19062 Colell, A., Garcia-Ruiz, C., Mari, M., and Fernandez-Checa, J. C., 2004, Mitochondrial permeability transition induced by reactive oxygen species is independent of cholesterol-regulated membrane fluidity, FEBS Lett 560, pp. 63-68... 

Figure 7.24. Mitochondrial respiration and membrane fluidity in differently acclimated congeners of abalone (genus Haliotis). (Top panel) Arrhenius break temperatures for mitochondrial respiration. Note overlapping data points (pinto at 5°C red and green at 20°C) have been offset by 1°C for clarity. (Bottom panel) Fluidity of mitochondrial membranes as estimated from the fluorescence polarization of DPH. Note 20°C points have been offset for clarity. (Data from Dahlhoff and Somero, 1993b.)...

MI-D (42) reduced the rate of swelling induced by valinomycin-K+, as well as induced swelling in the presence of nigericin-K+. Shrinkage was also affected, suggesting interference with the inner mitochondrial membrane, which would affect both the fluidity and elasticity. These alterations in the membrane fluidity are thus related to the effect of MI-D on the energy-linked functions of mitochondria [80]. 

Ricchelli, F., Gobbo, S., Moreno, G., and Salet, C. (1999) Changes of the fluidity of mitochondrial membranes induced by the permeability transition. Biochemistry 38(29), 9295-9300. 

Swelling Responses. Effects of the herbicides on the "fluidity" and permeability properties of membranes were measured. Alterations to the fluidity and permeability of organelle membranes can alter the osmotic properties of the membranes. Chloroplast, thylakoid, and mitochondrial membranes are known to be relatively impermeable to cations such as and IT , but freely permeable to lipophilic anions such as SCN (23,... 

Kolodziej, M.P. Zammit, V.A. (1990) Biochem. J., 272, 421-425. Sensitivity of inhibition of rat liver mitochondrial outer-membrane carnitine palmitoyltransferase by malonyl-CoA to chemical- and temperature-induced changes in membrane fluidity. 

It is largely accepted that a high dietary intake of poly-unsaturated fatty adds (PUFA) in the a>-3 series has beneficial effects. Recently, cellular lipid metabolism has been suggested as a target for cancer therapy. Cancer cells, compared with normal cells, seem to be vulnerable to exposure of certain polyunsaturated fatty acids (PUFAs), especially those in the o -3 series. Characteristic for these compounds are their poor abihty to be oxidized in the cell due to multiple double bonds. They are however likely to be ester-rfied to oflier Upids, and their incorporation into membrane phosphohpids will influence membrane properties such as fluidity, protein interactions and susceptibility to lipid peroxidation. The hypohpidemic properties of some (0-3 fatty acids, such as EPA, are probably e lained by an induction of mitochondrial P -oxidation that is not found after adrninistration of the non-hypolipidemic (o-3 PUFA docosahexaenoic acid (DHA)." However, both eicosapentaenoic acid (EPA) and DHA cause increased peroxisomal... 

References (21-23) describe time-resolved fluorescence anisotropy studies (using TMA-DPH and DPH as fluorescent probes), of the effect of immime complexes on macrophage membrane fluidity, the effect of succinate and phenyl succinate on the fluidity of the inner mitochondrial membrane, and the role of cholesterol in modulating the fluidity of renal cortical brush border and basolatered membranes, respectively. 

Plasma membranes isolated from paclobutrazol treated Agraveolens cells exhibited a reduced membrane fluidity and an accumulation of 14a-methylsterols(mainly obtusifoliol) accompanied by a decrease in phospholipid sterol ratio. In addition, the relative proportions of phosphatidylethanolamlne was reduced and that of phosphatidylserine was increased. A direct carboxylation of existing phosphatidylethanolamlne molecules could account for this latter observation [13]. In contrast, mitochondrial membranes exhibited an... 

Treatment of R rac/7/s cultures with paclobutrazol resulted in the accumulation of phosphatidate and 14a-methylsterols (particularly lanosterol and 24-methylenedihydrolanosterol) in both plasma and mitochondrial membranes. These changes may be directly related to the observed increase in membrane fluidity. Whether the accumulation of phosphatidate is a direct result of phospholipid retailoring mechanisms via phospholipase D activity is not yet known. 

A second possibility to account for the observed defect in DNA synthesis during growth at low unsaturated acid levels is that the decreased fluidity of the membrane under these conditions directly affects the process of mitochondrial DNA replication. It has been suggested that mitochondrial DNA synthesis is a membrane-associated process. Recent work in our laboratory has demonstrated that the fluidity of the mitochondrial membrane has a direct influence on mitochondrial DNA replication in vitro An Arrhenius plot of DNA replication in vitro has a discontinuity (Fig. 5), the temperature of which varies with the unsaturated fatty acid content of the mitochondrial membranes (Table II). These results demonstrate the existence of a close functional relationship between mitochondrial DNA replication and the physical properties of the mitochondrial membrane. 

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