Flow cytometry sperm

In immunolocalization studies of ciliated epithelium, Ron was detected in nasal and bronchial epithelium and in normal oviduct (Sakamoto et al., 1997). Primary cultures of normal human bronchial epithelium expressed Ron as assessed by flow cytometry and bound MSP with a Kd of 0.5 nM. MSP caused tyrosine phosphorylation of the receptor. Ron and Met were both expressed in ciliated bronchial epithelium, the latter in a basolateral location, the former at the apical surface just below the base of the cilia. This led to the finding that MSP caused a transient increase of about 20% in the ciliary beat frequency of nasal mucosal cells. In view of Ron mRNA expression in sperm and MSP mRNA in epithelium of epididymis (Ohshiro et al., 1996), the authors suggest a possible role for MSP in sperm motility as well as in mucociliary transport. 

Perreault, S, D, Barbee, R. R-. Elstcin, K, H Zucker, R, M and Keefer, C, L, (1988). Intcrspccies differences in the stability of mammalian sperm nuclei as,scsscd in vivo by sperm mieroin-jeciion and in vitro by flow cytometry, Bioi. Reprod. 39, 157-167. 

Evenson DP, Emerick RJ, Jost LK, et al. 1993. Zinc-silicon interactions influencing sperm chromatin integrity and testicular cell-development in the rat as measured by flow cytometry. J Animal Science 71(4) 955-962. 

Only a few representative applications have been cited above. In addition it may be said that flow cytometry finds numerous applications in the field of cell differentiation, immunology, parasitology, sperm analysis, food science, pharmacology and toxicology, cancer oiology and carcinogenesis, bone marrow analysis, tissue typing and lymphocyte applications, T-cell subset analysis. 

Matsumoto, T. Okada, H. Hamaguchi, Y. Method and reagent for counting sperm by flow cytometry. Jpn. Kokai Tokkyo Koho JP 2001242168, 2001 Chem. Abstr. 2001, 135, 207869. 

Troiano, L. Granata, A. R. M. Cossarizza, A. Kalashnikova, G. Bianchi, R. Pini, G. Tropea, F. Carani, C. Franceschi, C. Mitochondrial membrane potential and DNA stainability in human sperm cells a flow cytometry analysis with implications for male infertility. Exp. Cell Res. 1998, 241, 384-393. 

I. Erenpreisa, J. Spano, M. Toluidine blue cytometry test for sperm DNA couformation comparison with the flow cjdometric sperm chromatin structure and TUNEL assays. Hum. Reprod. 2004, 19, 2277-2282. 

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