Model fibrinogen interaction

Rose, T., and Di Cera. E, 2()02. Three-dimensional modeling oi thrombin-fibrinogen interaction, y. Biol C/ieni. 277 18875 ]8tS,W... 

The crystal structures of fragment D from several recombinant fibrinogens has demonstrated that these mutant molecules are excellent models for the study of structure-function relationships, since the polypeptide chains are folded properly with only local changes (Kostelansky et al., 2002). Moreover, these studies allow testing of hypotheses about the effects of a particular mutation on structure and internal interactions within the molecule, which then can be correlated with the effects on fibrin polymerization or platelet aggregation (Kostelansky et al., 2004a,b). 

We have shown that the morphology of the adsorbed individual molecule and the protein film is dependent upon the surface to which the protein is adsorbed and the wall shear rate at which the protein solution contacts the surface. We have studied the interaction of proteins with surfaces from two approaches the first involved observations of the tertiary structures of individual molecules, and the second involved the use of a flow cell and the exposure of test materials to flowing, dilute, purified human plasma protein solutions. On the hydrophobic surfaces of polystyrene and polycarbonate, albumin and fibrinogen films form networks. On the surface of the hard segment model polyurethane, the morphology of the adsorbed film is very similar to that formed on the hydrophobic polystyrene and polycarbonate surfaces. On the peu-2000, the lowest... 

The next step in neutrophil extravasation is inhibited by the Extracellular adherence protein (Eap) of S. aureus. Eap inhibits direct interactions with the host adhesive proteins intercellular adhesion molecule 1 (ICAM-1), fibrinogen or vitronectin, Eap disrupted beta(2)-integrin and urokinase receptor mediated leukocyte adhesion in vitro. In a bacterial peritonitis mouse model, Eap-expressing S. aureus (or isolated Eap) elicited less neutrophil influx as compared to an Eap-negative strain. - Further Eap protects in an ICAM-1 dependent experimental autoimmune encephalomyelitis (EAE) in mice, indicating that Eap could possibly represent a treatment... 

FIGURE 6.19 The size distributions of pores filled by unfrozen water for (a) aqueous suspension of A-300 and the PSD calculated on the basis of the nitrogen adsorption/desorption isotherm (with the model of voids between spherical particles) (b) 0.15 mol NaCl or HPF solution at 1.25 and 2.5 wt% and (e) HPF/A-300 at different concentrations of protein and siliea. (Adapted with kind permission from Springer Seience+Business Media Cent. Eiir. J. Chem., Interaction of fibrinogen with nanosilica, 5, 2007, 32-54, Rugal, A.A., Gun ko, V.M., Barvinchenko, V.N., Turov, W., Semeshkina, T.V., and Zarko, V.I.)... 

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