Ferric ammonium citrate solution

Dissolve the ingredients separately in water. Mix the ferric ammonium citrate solution and the tartaric acid solution then add the silver nitrate solution while stirring with a nonmetal rod. Add water to make up the complete quantity. Keep in a brown glass bottle, away from light. 

Theory In ferric ammonium citrate it is taken for granted that the entire iron is oxidized to the Fe2+ state and practically little Fe2+ is present. Thus, the ferric ion present in a known amount of the sample liberates an equivalent amount of iodine from an acidified KI solution. Thus, we have ... 

Materials Required Ferric ammonium citrate 0.5 g sulphuric acid cone. 1 ml 0.1 N KMn04 solution 50 ml hydrochloric acid 15 ml potassium iodide 2.0 g 0.1 N sodium thiosulphate. 

Procedure Weigh accurately about 0.5 g of ferric ammonium citrate and dissolve the sample in 15 ml DW. Add to it slowly 1 ml of sulphuric acid and warm gently to attain a yellow colouration so as to decompose the iron and ammonium citrate complex completely. Cool and add 0.1 N potassium permanganate solution dropwise from a burette to obtain a pink colour that persists for 5 seconds. To the resulting solution add hydrochloric acid 15 ml and potassium iodide 2.0 g, shake well and set aside for 3 minutes so that iodine may be liberated completely. Now, add 60 ml of water and titrate with 0.1 N sodium thiosulphate solution while shaking the contents continuously till a colourless end-point is achieved. 

Compound Name Ammonium Hydroxide Hexamethylenetetramine Ammonium Acetate Ammonium Bifluoride Ammonium Sulfamate Ammonium Sulfamate Ammonium Benzoate Ammonium Bicarbonate Ammonium Dichromate Ammonium Bifluoride Ammonium Carbonate Ammonium Chloride Ammonium Citrate Ammonium Citrate Ammonium Pentaborate Ammonium Dichromate Nickel Ammonium Sulfate Ferric Ammonium Citrate Ferric Ammonium Oxalate Ferrous Ammonium Sulfate Ammonium Fluoride Ammonium Silicofluoride Ammonium Formate Ammonium Gluconate Ammonium Bicarbonate Ammonium Bifluoride Ammonium Sulfide Ammonium Hydroxide Ammonium Thiosulfate Ammonium Thiosulfate Ammonium Iodide Ferrous Ammonium Sulfate Ammonium Lactate Ammonium Lactate Ammonium Lauryl Sulfate Ammonium Molybdate Ammonium Chloride Nickel Ammonium Sulfate Ammonium Nitrate Ammonium Nitrate-Urea Solution Ammonium Oleate... 

Uses In iron-toning solutions. Fresh ferric ammonium citrate tends to form a layer of mold after a few days. This can be filtered or lifted off. After the first time the mold rarely returns. Notes Both brown and green salts are light-sensitive. Keep well closed and protected from light. 

Distilled water at 125F/52C, 500.0ml Ferric ammonium citrate, 8.0g Potassium ferricyanide, 8.0 g Acetic acid, 28% solution, 265.0ml Distilled water to make 1.0 liter... 

Figure 3. The inhibition of E. coli around wells charged with human serum or Tr and the neutralization of microbiostasis on serum medium around wells charged with a solution of ferric ammonium citrate (20 i g iron/well) or spent medium at 15-, 25-, and 35-hr incubation period (25)...

Ferric Ammonium Citrate, Brown, occurs as thin, transparent brown, red-brown, or garnet red scales or granules, or as a brown-yellow powder. It is a complex salt of undetermined structure, composed of iron, ammonia, and citric acid. It is very soluble in water, but is insoluble in alcohol. The pH of a 1 20 aqueous solution is about 5.0 to 8.0. It is deliquescent in air and is affected by light. 

Ferric ammonium citrate (C6H607Fe.C6H60r(NH4)2H), is used in making blue-print paper (242). A strong solution of neutral ammonium citrate is used in the analysis of fertilizers in the separation of the different forms of phosphates which the fertilizers contain. 

Details of the TIBC assay differ slightly from assay to assay. Typically, 1 mL of serum is mixed with 0.1 mL ferric ammonium citrate (1.25 mmol Fe /liter) for 10 min after which 2 mL barbital-NaCl buffer and 0.4-0.5 g of magnesium carbonate (light powder) are added. Mix for 15 min on a rotary mixer after capping with parafilm. Centrifuge for 10 min at ISOOxg remove 2 mL of the supernatant solution, and spectrophotometrically analyze for iron as described above. Correction for the serum dilution (3 1) is included in calculating the TIBC. 

Citric acid 2 g. ferric ammonium citrate 0.05 g. K2HP04 0.5 g. glycerol 20 g. glutamic acid or asparagine 4 g. 5 ml. 10 % MgS04 solution, adjusted to pH 7.4 with ammonia. The final volume is brought to 3 1. with distilled water and sterilized in the autoclave. 

Stock mineral solution ZnS04 7 H2O, 2.8 g/1 ferric ammonium citrate, 2.7 g/1 ... 

The resulting salt, whilst readily soluble in dilute mineral acids, is insoluble in cold acetic acid, phosphoric acid, and sodium phosphate. It is slightly soluble in citric and tartaric acid solutions, and readily dissolves m neutral aqueous ammonium citrate, yielding a green solution with a brownish tint.3 The salt is insoluble in water, but hot water hydrolyses it, and boiling with excess of ammonia solution converts it into a mixture of ferric hydroxide 4 and ferric phosphate, or if the ammonia is present in great excess the ferric phosphate may be entirely decomposed. Thus —... 

Citrates.—Ferric Citrate—Ferri citras (XT. S.)—(Fea)(C H60T)a- -6Aq—489.8-1-108—is in garnet-colored scales, obtained by dissolving ferric hydrate in solution of citric acid, and evaporating the solution at about C0° (140° F.). It loses 3 Aq at 120° (248° F.), and the remainder at 150° (302° F.). If a small quantity of ammonium hydroxid be added, before the evaporation, the product consists of the modified citrate = ferri et ammonii citras (XT. S.), which only reacts with potassium ferrocyanid after addition of HCl. 

---