Feasibility of the Induced Circular Dichroism Technique for Nucleic Acid Research

2 Feasibility of the Induced Circular Dichroism Technique for Nucleic Acid Research 

Neville and Bradley 79) were first to report that DNA induced Cotton effects in the optical rotatory dispersion spectrum of acridine orange. The investigation of DNA-induced Cotton effects has been extended to other aminoacridines. According to Peacock 80), aminoacridines may be divided into two groups. The DNA-induced ICD of the first class (aminoacridines having a 3-amino group) increases steeply and cooperatively with increasing amounts of bound aminoacridine /% where r is 

Therefore, the ICD method should be applied to the analysis of the DNA-dye complexes together with other physicochemical structural analyses such as NMR spectroscopy or X-ray crystallographical analysis. 

In Sect. 4.1 it was described that the left-handed Z form of double-strand poly(dG-m5dC) poly(dG-m5dC) is formed at much lower salt concentrations than for the unmethylated copolymer. The B-Z transformation has been investigated with 31P NMR 84), IR 168), and CD 85) spectroscopies. Adriamycin (Formula 20) is a cytotoxic 

Poly(8-methyladenylic acid) can form a regular, single-strand helix, the first such structure found to exist in aqueous solution. In this polymer, the glycosidic conformation is a regularly alternating syn-anti structure. It is interesting that the alternation of syn and anti conformations may be correlated with Z-DNA formation and that the bulky 8-methyl group is expelled to the outside of the stack s array of bases 171). 

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