FCS fetal calf serum

A. Collagen substrate, 0% FCS (fetal calf serum) B. Spider silk-ELP substrate, 0% FCS C. No substrate, 0% FCS D No substrate, 10% FCS. In A and C, the cells have an elongated, fibroblastoid morphology, which characterizes dedifferentiation. The spider silk-elastin substrate (B) or the presence of 10% FCS in dishes without a substrate (D) produce a more favorable morphology. 

FCS fetal calf serum hTERT human telomerase catalytic subunit... 

FCS)(PBS phosphate buffered saline FCS fetal calf serum) was added, incubated overnight at 4 C, and the first wash consisted of PBS/Tween. Binding of the DES to the well was maximized to 46% with the Tween wash. Anti-DES antibody (rabbit IgG) was added at a concentration of 1 900, incubated at 37 C for one hour, washed with PBS, and followed with alkaline phosphatase conjugated anti-rabbit IgG (Sigma) at a concentration of 1 3000. This was incubated for one hour at 37 C, washed with PBS, and followed with p-nitrophenyl phosphate substrate (FNP)(1 mg/ml) in diethanolamine buffer (pH 9.6). After 45 minutes in darkness, an average positive... 

Cultures. Swiss 3T3 and A431 cells were grown in a gassed (5.5% COJ, humidified incubator in Dulbecco s Modified Eagle Media (DME) supplemented with 10% heat inactivated fetal calf serum (FCS). 

Figure 5 IL-6-inducing activity of HGLs in THP-1 cells in the absence (a) and presence of fetal calf serum (FCS) (b). The data present one of several independent experiments with similar results. The IL-6 induetion by unstimulated eells was less than 50 pg/mL. Eaeh bar represents the means SD.

Post-HIER immunostaining buffer 0.05 M Tris-buffered saline (TBS, pH 7.6) containing 1-2% fetal calf serum (FCS) or normal goat serum. 

Protein adsorption is the first event that takes place on material surfaces when blood or other body fluids are brought into contact with any material. Therefore, cell - material interactions must be discussed by taking into consideration the species and the nature of the protein adsorbed on the material surfaces. For instance, a series of cell-attachment and spreading experiments [11] of fibroblasts on the surface of modified polystyrene (TCP and Primaria) carried out in the presence of fetal calf serum (FCS) showed that FCS contains components which tend to decrease the attachment and spreading of fibroblast cells. The effect of these nonadhesive components was only evident when the FCS was depleted of vitronectin, showing that vitronectin overcomes the effect of these nonadhesive components and promotes cell-attachment and spreading on the polystyrene surface. Fibronectin, on the other hand, does not play a principal role in this fibroroblast adhesive process (Fig. 2). 

Grow the fibroblasts in Ham F-10 medium with 10% fetal calf serum (FCS). Harvest the cells, seed at a density of 30,000 cells and grow to near confluency on cover slips in 6-well plates in Ham F-10 medium with 10% FCS. Remove the medium and wash the cells three times with 2 ml Hanks balanced salt solution. Fix the cells with 3 ml fixative. Seal the plate with tape and store in the refrigerator at 4 C until staining. 

Fetal calf serum (FCS) inactivated by heating for 30 min at 5-6°C and tested for ability to support growth of hybridomas (see Note 5). 

Fetal calf serum (FCS) for bioreactor medium (see Note 6). Batch test before purchase (see Note 7). Inactivate the complement by heating to 56°C for 30 mm and store in 100-mL aliquots at -20°C... 

Reagents. The following buffers and solutions are needed for whole cell screening growth media Dulbecco s modified Eagle s medium (DMEM), 10% fetal calf serum (FCS), 100 units/ml penicillin, and 100 /ig/ml streptomycin, PBS 8 mM Na2HP04, 1.4 mM KH2P04, 137 mM NaCl, 2.7 mM KC1, pH 7.2. 

Cell culture medium for PBMCs, RPMI1640 (Gibco-BRL) containing 20% fetal calf serum (FCS), 60 pg/mL penicillin, and 100 pg/mL streptomycin. Supple-... 

Seed 50,000 cells/well in 48-well cell culture plates in DMEM with 10% fetal calf serum (FCS) 24 h prior to ligand incubation. 

Williams Medium E, containing 5% fetal calf serum (FCS). 

Leibowitz culture medium plus 10% fetal calf serum (L-15 plus FCS). 

Table 2 summarizes the results of M<[) phagocytosis of cellulose microspheres whose surfaces were grafted with various protein molecules, together with the microspheres not grafted or modified by coulping with a -hexyl (Q) chain, as well as polystyrene microspheres. In addition, the effect of fetal calf serum (FCS) added to M<[) cultures... 

Fetal calf serum (FCS) or another convenient protein supplement (for instance 4% bovine serum albumin [BSA] solution). Store sterile at 4°C or for periods longer than 1 mo at -20°C. 

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