Fast-scan cyclic voltammetry FSCV

On the contrary, the radical cation of anthracene is unstable. Under normal volt-ammetric conditions, the radical cation, AH +, formed at the potential of the first oxidation step, undergoes a series of reactions (chemical -> electrochemical -> chemical -> ) to form polymerized species. This occurs because the dimer, tri-mer, etc., formed from AH +, are easier to oxidize than AH. As a result, the first oxidation wave of anthracene is irreversible and its voltammetric peak current corresponds to that of a process of several electrons (Fig. 8.20(a)). However, if fast-scan cyclic voltammetry (FSCV) at an ultramicroelectrode (UME) is used, the effect of the follow-up reactions is removed and a reversible one-electron CV curve can be obtained (Fig. 8.20(b)) [64], By this method, the half-life of the radical cat-... 

Fast-scan cyclic voltammetry (FSCV) is being extended into biomedical fields, particularly in the areas of neurophysiology and interdisciplinary psychoanalytical... 

The slice is electrically stimulated with an enamel-coated bipolar wire electrode causing action potentials that evoke DA release. In the slice preparation, the stimulation is applied directly at the neuron terminals. This is in contrast to an in vivo experiment, where the stimulation is performed at the cell body of the neuron and the DA release is monitored remotely at the presynaptic terminals. DA concentration in the extracellular fluid rises and quickly returns to baseline at the cessation of the stimulation [3]. Fast scan cyclic voltammetry (FSCV) at carbon-fiber microelectrodes is used to detect the resulting concentration changes in the extracellular fluid. This analytical technique provides a method for the determination of uptake kinetics in intact brain tissue. Thus, the secretion and subsequent clearance of DA in the tissue is observed in real time. 

The use of SECM to probe homogeneous reaction kinetics can be traced to the earliest applications of UMEs to profile concentration gradients at macroscopic (millimeter-sized) electrodes. There has been considerable progress subsequently, such that short-lived intermediates, in electrode reactions, can readily be identified by SECM under steady-state conditions, that would be difficult to characterize by alternative transient UME methods, such as fast scan cyclic voltammetry (FSCV). 2... 

The coupling of fast scan cyclic voltammetry (FSCV) with SECM to resolve the distribution of chemical species generated at a substrate surface was first introduced by Wipf s group [169,170]. In FSCV-SECM, CV is carried out at an SECM tip using rapid scan rates, typically between 10 and... 

FSCV-SECM Fast scan cyclic voltammetry scanning electrochemical microscope... 

Because of its small size, selectivity, time response, and sensitivity, the carbon fiber microelectrode coupled with fast-scan cyclic voltammetry currently represents the most ideal sensor for the measure of kinetics and mechanisms of DA neurotransmission. Release and uptake sites (neuron terminals) are depicted in Figure 3 along with a typical example of DA release and uptake data monitored with a carbon-fiber microelectrode using FSCV. These sites are generically described as a cartoon merely to give the reader an idea of the relative size of the electrode versus the neuron terminals (release and uptake sites). 

FLD = fluorescence detection, LIF = light induced fluorescence, LOD = limit of detection, LC = liquid chromatography, ECD = electrochemical detection, PFET = photoluminescence following electron transfer, MS/MS = tandem mass spectrometry, CE = capillary electrophoresis, FSCV = fast-scan cyclic voltammetry. 

---